Objective To study hepatic growth factor（HGF）and c-met expressions in rat models with liver failure and partial hepatectomy.Methods Liver failure induced by intraperitoneal injection of LPS/D-GalN and by 70% partial hepatectomy was established，and healthy rats served as controls.The level of serum HGF was detected by ELISA assay and c-met gene expression were detected by real-time PCR and Western-bloting.Results Compared with the control group，the serum HGF level and its receptor c-met were significantly elevated in rats with partial hepatectomy，however，the serum HGF level increased with c-met expression significantly reduced in rats with liver failure.Conclusion The compromised hepatic c-met expression might be essential for defects in HGF-mediated liver regeneration in rats with liver failure.

Objective To explore the effect of different dosages of arginine on serum cytokines in rats with acute hepatic failure.Methods 60 healthy Wistar rats were randomly divided into control（group A），hepatic failure （group B），arginineⅠ（group C），arginineⅡ（group D），arginineⅢ（group E） and arginineⅣ（group F）.The hepatic failure was induced by D-galactosamine.Results The level of TNF-α in all groups raised except A group;there were significant differences between group B，C and group A（P<0.05）;The level of IL-2 and IL-10 in all groups decreased except group A;there were statistically significant differences between group B，C and group A （P<0.05）.Conclusion Supplement of arginine could up-regulate the levels of some cytokines in rats with acute hepatic failure.The dosages of 0.8g.kg-1.d-1 and 1.6g.kg-1.d-1 are more effective than any other dosages.

Objective To explore the anti-oxidant effects of silymarin on intestinal tissues in rats with non-alcoholic steatohepatitis.Methods Fifty Wistar rats were randomly divided into control，model and silymarin groups.The rats in control group were fed with normal diet.The rats in model group were fed with fat rich diet and saline by gastric lavage Methods to establish the model of NASH.The rats in silymarin group were fed with fat rich diet and silymarin by gastric lavage method.All rats in each group were observed for 12 weeks.Results The serum levels of TC，TG，ALT and AST were higher in model group than those in control group.The serum levels of TC，TG，ALT and AST were lower in silymarin group than those in model group（F=36.626～64.327，P<0.01）; The activitives of SOD were lower in model group than in control group（83.29±10.32 U/mgprot vs.52.34±7.09 U/mgprot）.The activitives of SOD in silymarin group were higher than those in model group; The changes of MDA were on the contrary（0.56±0.07 Nmol/mgprot vs.0.97±0.09 Nmol/mgprot，F=23.488 and 10.609，respectively，P<0.05）;The levels of ET in model group（0.316±0.020EU/ml）were higher than in silymarin grou p（0.279±0.022EU/ml）.Conclusion Silymarin，an anti-oxidant drug，can protect the hepatocytes and the intestines in rats with NASH.

Objective To investigate the changes of blood neutrophils and platelets in patients with chronic hepatitis B during the use of ordinary IFN-α -2b treatment.Methods 24 patients with chronic hepatitis B receiving ordinary IFN-α-2b treatment were followed-up for 48 weeks.Result After the use of IFN-α-2b，the obvious decrease of neutrophil and platelet counts appeared in the second week and fourth week，respectively，and both of them recovered gradually thereafter.Conclusion The decrease of neutrophils and platelets in blood during the use of ordinary IFN-α-2b is reversible and the outcome is good.

Objective To explore the change and clinical significance of coagulation function index and platelet’s parameters in patients with chronic hepatitis B.Methods 42 severe chronic hepatitis，24 acute-on-chronic liver failure，35 chronic liver failure，32 liver cirrhosis and 50 healthy peoples as control were collected. Their coagulation function index（PT，APTT，TT，Fig） and platelet’s parameters（PLT，MPV，PDW，P-LCR） were detected.Results Compared with healthy group，MPV，PDW，P-LCR increased and PLT descended in various types of chronic hepatitis groups（P<0.01）;MPV，PDW，P-LCR were higher and PLT were lower in acute-on-chronic liver failure， chronic liver failure and liver cirrhosis groups than severe chronic hepatitis group（P<0.01）;PLT，MPV，PDW in acute-on-chronic liver failure group and MPV in chronic liver failure group were higher than those in liver cirrhosis group（P<0.05，P<0.01）;There was significant difference in PLT between acute-on-chronic liver failure and chronic liver failure groups（P<0.01）;PT，APTT，TT were higher and Fig were lower in acute-on-chronic liver failure，chronic liver failure and liver cirrhosis groups than severe chronic hepatitis group（P<0.01）;Fig were lower in acute-on-chronic liver failure and chronic liver failure groups and APTT was higher in chronic liver failure group than those in liver cirrhosis group（P<0.01）.There was significant difference in APTT between acute-on-chronic liver failure and chronic liver failure groups（P<0.01）.Conclusion Coagulation and platelet parameters can reflect the degree of liver damage and bleeding tendency in patients with chronic hepatitis B.

Objective To explore the relationship between liver fibrosis stages /inflammation grades and blood platelet counts and serum fibrosis indices in patients with chronic hepatitis B.Methods The liver fibrosis stages /inflammation grades in 136 CHB patients were confirmed by pathological examination using biopsy tissues. The blood platelet counts and serum fibrosis indices（pro-collagen III，collagen IV，hyaluronic acid，laminin） were tested in the patients.Results Significant negative correlations were found between liver fibrosis stages /inflammation grades and blood platelet counts （Spearman r= -0.370 and -0.356，P=0.002 and 0.0003，respectively）;However，significant positive correlations were found between the liver fibrosis stages /inflammation grades and liver fibrosis indices（Spearman r for PC-III，C-IV，HA，and LN were 0.414，0.390，0.419，0.393，and 0.426，0.375，0.548，0.343，respectively，all P<0.05）.Conclusions The blood platelets，PC-III，C-IV，HA，and LN may be used as an noninvasive markers for liver injuries in CHB patients.

Objective To evaluate the efficacy of adefovir dipivoxil（ADV） on patients with chronic hepatitis B，and its association with HBV genotypes.Methods 75 patients with chronic hepatitis B were treated with ADV for 48 weeks，and the HBV genotypes were detected.Results In all patients，HBV genotype B，C and B/C were 49（65.4%），22（29.3%） and 4（5.3%），respectively;ALT normalization and HBV DNA negative rates at week 48 were 58.7% and 50.7%，HBeAg loss and HBeAg seroconversion rates were 34.9% and 1.5%，respectively;The ALT normalization and HBV DNA negative rate in genotype B group at week 12 were 26.5% and 38.8%， which came to 55.1% and 53.1% at week 48，respectively，while in genotype C group at 12 week were 13.6% and 31.8%，which came to 59.1% and 36.4% at 48 week，respectively.Conlusions The baseline HBV DNA levels affect the antiviral response to ADV，especially in patiens with HBV DNA>1×105copies/ml.There was no association between the efficacy of adefovir dipivoxil and HBV genotypes.

Objective To study the related factors of a sustained virologic response in patients with chronic hepatitis C genotype 1.Methods A total of 73 de novo chronic hepatitis C genotype 1 patients received combination therapy of peginterferon alpha and ribavirin for 24 to 48 weeks and were followed-up for 24 weeks.Results 47.9% of patient achieved an early viral response and 38.4% of patients got an sustained viral response;Statistical analysis indicated that patients with lower baseline HCV load（<8×105 IU/ml），lower body mass index and early viral response would achieve sustained viral response（SVR）.Conclusion Baseline HCV load， BMI and early viral response are related to achieving SVR in de novo hepatitis C genotype 1 patients.

Objective To investigate the effects of ornithine aspartate granules on the level of serum gamma-glutamyltransferase（GGT） in patients with chronic liver diseases.Methods The patients with high level of serum GGT with chronic liver diseases were treated by oral ornithine aspartate granules while the control group were treated by oral bicyclol tablets for 30 days.Results The GGT level before and after treatment were 196.98±190.80 U/L and 96.10±89.84 U/L in ornithine aspartate treatment group（P＜0.01），while the GGT level were 168.06±87.10 U/L and 117.30±52.63 U/L in bicyclol treatment group（P＜0.05），respectively.However，the GGT leves in both groups was not significantly different.The GGT levels were positively related to the ALT levels（P＜0.001）in both groups.Conclusions Ornithine aspartate granules and bicyclol tablets can reduce serum GGT level and improve inflammation of the liver damage in patients with high level of serum GGT with chronic liver diseases.

Objective To explore the effect and safety of autologous bone marrow stem cell transplantation via hepatic artery in treatment of patients with decompensated cirrhosis.Methods 425 patients with decompensated liver cirrhosis were selected for the trail.Beside the conventional treatment，the bone marrow stem cells were transplanted into the liver via hepatic artery.The biochemical index of liver function，abdominal B ultrasound and liver CT were detected at different times after transplantation.Results There was no severe side effects in all the 425 patients after the operation. 2 weeks after transplantation，abdominal distention was alleviated in 213 patients，8 weeks after transplantation，appetite was improved in 297 patients.12 weeks after transplantation，ALB increased from 26.6±5.2g/L to 37.5±4.9g/L（P<0.05），ALT decreased from 150.7±19.5U/L to 30.7±7.7U/L（P<0.05）， AST decreased from 85.6±32.5U/L to 20.5±15.7 U/L（P<0.05），TBIL decreased from 154.4±20.2μmol/L to 52.3±17.4μmol/L，PT decreased from 20.1±1.7s to 15.4±1.5s. Conclusion Transplantation of autologous bone marrow stem cells via hepatic artery is safe and effective in the treatment of patients with decompensated cirrhosis.

Objective To evaluate the relationship of polymorphism of complement C5 gene and occurrence of liver cirrhosis in patients with chronic hepatitis B.Methods Polymorphism of complement C5 gene rs17611 was examined in 182 Chinese patients with chronic hepatitis B or liver cirrhosis by using TaqMan PCR genotyping method.Results Patients with genotypes G/G，A/G and A/A were detected with A/A genotype higher in patients with liver cirrhosis（P=0.022）;By multivariate logistic regression analysis，genotype A/A was an independent factor associated with liver cirrhosis （odds ratio=5.1，P=0.008）.Conclusion Polymorphism of complement C5 gene rs17611 is associated with progression of liver disease in Chinese patients with hepatitis B virus infection.

Objective To explore the application of three enhanced scanning and three-dimensional reconstruction of 64-slice spiral CT in the diagnosis of extrahepatic bile duct carcinoma.Methods 20 patients with surgically and pathologically proved extrahepatic bile duct carcinoma were examined by 64-slice spiral CT.Results Out of 11 patients with hilar cholangiocarcinoma，the tumors located in the left hepatic duct in 3，in right hepatic duct in 2 and in hepatic duct terminal in 6 cases;Intrahepatic bile ducts were dilated in 11 cases with hilar cholangiocarcinoma;mass enhancement was not obvious in early phase;in delayed phase，9 cases showed complete enhancement，and heterogeneous enhancement in 2 cases;Dilated common bile duct was suddenly interrupted or deformed in 9 cases with cholangiocarcinoma of common bile duct;Intraductal soft tissue node was shown in 2 cases.Conclusion 64-slice spiral CT is an ideal Methods to diagnose the extrahepatic bile duct carcinoma.

Objective To investigate the clinical effects of transcatheter arterial chemoembolization （TACE） combined with three dimensional conformal radiotherapy（3-DCRT）on patients with advanced hepatocellular carcinoma.Methods A total of 87 patients with advanced hepatocellular carcinoma were randomly divided into TACE/3-DCRT group（n=45）and TACE group （n=42）.The patients in TACE/3-DCRT group were treated first with TACE and then 3-DCRT，the dose was 2.6～3.2Gy each time， and the total dose was 48～60Gy. The patients in TACE group were given TACE only.Results The total response （CR+PR） were 86.7% in TACE/3-DCRT group， much higher than 66.7% in TACE group（P<0.05）;The 1，2，3-year survival rates were 73.3%，53.3%，35.6% and 59.5%，31.0%，14.3%，respectively in the two groups.Conclusions TACE combined with 3-DCRT is more effective than TACE only，and the side effects are tolerated.

Objective To explore the characteristic and related affecting factors to hospitalization fee in patients after liver transplantation.Methods Hospitalization fee of 84 patients who undergone liver transplantation in several years were categorized and then statistically studied retrospectively.Results The average hospitalization fee of 84 patients was 23235.20 yuan and the top 3 costs in hospitalization fee was medicine（66.61%），check-up（18.02%）and bed occupation（7.45%），respectively;The majority of patients was retired and had some severe complications.Conclusions It is important to prevent and control the severe complications for patients after liver transplantation in order to decrease the hospitalization fee.