Objective To investigate the anti-proliferation effects of cell-permeable TAT-N24 fusion peptide in hepatoma cell line． Methods HepG2 cells were treated with cell-permeable TAT-N24 fusion peptide． The cell cycle was analyzed by flow cytometry，the proliferation of the cells was assayed by BrdU incorporation and the expression of phosphorylated AKT was detected by Western blot. Results The incorporation rates of BrdU in blank，control and TAT-N24 group were 42.7±3.6%，38.2±2.8% and 25.3±2.7%（P<0.05），respectively；the percentages of G0/G1 phase cells were 52.6±4.7%，52.0±4.6% and 68.6±4.7%（P<0.05），respectively；the phosphorylation of AKT in the three groups was not significantly different. Conclusion Cell-permeable TAT-N24 fusion peptide effectively inhibites the DNA synthesis and induces cell cycle blocked at G0/G1 phase of HepG2 cells.

Objective To investigate the effects of L1 encoded ORF-1p on the proliferation of SMMC7721 cells. Methods Recombinant expression vector pcDNA3.0-Flag-ORF1 was used to transfect SMMC7721 cells;Expression of ORF1p in the transfected cells was detected by Western blotting;Proliferation and anchor-independent growth of the cells transfected with Flag-ORF1p vector were detected by MTT assays and soft-agar anchored colony formation assays;Transcriptional activity of p15，p21 and p53 reporter genes was analyzed by luciferase reporter system. Results Flag-ORF-1p could enhance the proliferation and anchor-independent growth ability of SMMC7721 cells;It also reduced the transcriptional activity of p15，p21 and p53 luciferase report genes. Conclusion ORF-1p of L1 might enhance the SMMC7721 cell proliferation and anchor-independent growth through regulation of some genes.

Objective To elucidate the impact of hepatitis B virus rtN238H variant on adefovir resistance. Methods The rtN238H incidence and its relationship with adefovir（ADV）administration were analyzed in 1789 patients with chronic hepatitis B. The rtN238N variant from a representative case was cloned and its wild-type counterpart was acquired by reverse site-directed mutagenesis. pTriEx-HBV1. 1 vetors harboring N238H variant or wild-type strain were respectively constructed and transfected into HepG2 cells. The cells were treated with serial concentration of ADV. After digesting uncoated DNA with DNase，HBV DNA in viral particles in culture supernatant was released and quantitated by real-time PCR. Results One hundred and eighty one（10.1%）patients were identified as having rtN238H，out of them，151（83.4%）were genotype B，and 30（16.6%）were genotype C；130 ADV-treated patients（71.8.%）had higher incidence of rtN238H variant than 51 ADV-untreated patients（28.2%）；However，compared to wild-type strain，rtN238H had similar sensitivity to ADV and viral replication competence in vitro. Conclusion The rtN238H is a naturally-developed polymorphism without direct impact on ADV sensitivity and viral replication.

Objective The aims of this study are to clarify the relationships among the serum alpha-fetal protein（AFP） levels and liver histological activity index and hepatic fibrosis stages，as well as the status of HBeAg in patiemts with chronic hepatitis B. Methods The serum AFP levels were detected in 189 patients with chronic hepatitis B who had liver biopsies. Results The serum AFP levels in patients with hepatic histological inflammation grade 1，2，3and 4 were 4.09±1.94μg/L，6.51±3.42μg/L，19.71±23.72μg/L and 33.88±24.29 μg/L in HBeAg positive chronic hepatitis B，and 3.99±2.65μg/L，6.74±4.22μg/L，22.21±23.83μg/L and 40.02±45.32 μg/L in HBeAg negative group，respectively；The serum AFP levels in hepatic fibrosis stageⅠ，Ⅱ，Ⅲand Ⅳwere 5.28±3.00μg/L，6.97±3.46μg/L，18.16±20.26μg/L and 35.06±28.56μg/L in HBeAg positive chronic hepatitis B，and 4.89±3.20μg/L，5.62±3.40μg/L，19.51±23.00μg/L and 32.89±37.88μg/L in HBeAg negative group，respectively. Conclusions The mild increases of serum AFP levels are related to liver histological inflammation grades and fibrosis stages but not the status of HBeAg during the natural history of chronic hepatitis B.

Objective To observe the clinical effects of herbal medicine in patients with chronic hepatitis B. Methods Fifty patients with hepatitis B were treated with herbal medicine for 6 months. Results After treatment，the serum HA，PCIII，IV-C and LN decreased（P<0.01），and the spleen thickness and portal vein diameter narrowed（P<0.05）；the liver function index were improved obviously（P<0.01）. Conclusion The herbal medicine has apparent curative effect on hepatic fibrosis，and can improve hepatic function.

Objective To explore adefovir dipivoxil and bicyclol in treating patients with chronic hepatitis B. Methods 62 patients with chronic hepatitis B were treated with adefovir dipivoxil and bicyclol for 96 weeks，and 60 were with adefovir dipivoxil for 96 weeks. Results At the end of the 96 week treatment，the ALT normalization rate in combination group was 96.8%，much higher than in control（80.0%，x2=5.8，P<0.01）；the HBeAg（41.9% vs.33.3%，x2=2.1，P>0.05）and HBV DNA loss（85.5% vs.73.3%，x2=0.6，P>0.05）in the two groups were not significantly different；the serum HA，LN，PC Ⅲ and Ⅳ-C levels in combination group decreased more obviouly than in control（P<0.01）. Conclusion Adefovir dipivoxil combined with bicyclol can improve liver function，and decrease serum markers of liver fibrosis.

Objective To report the clinical features of patients with coagulation factor Ⅶ defciency and hepatitis B. Method One patients with chronic hepatitis B and coagulation factor Ⅶ defciency was reported.Result The patients had a prolonged PT and normal APTT，while he had a positive serum hepatitis B markers and normal liver function tests. Conclusion Coagulation factor Ⅶ defciency is a rare hemorrhage disease with some gene defect. A patient with prolonged PT and normal liver functions should be diagnosed carefully.

Objective To investigate whether the level of viremia differs in accordance with genotypes and responses to pegylated interferon and ribavirin treatment in patients with chronic hepatitis C. Methods 113 patients with chronic hepatitis C were treated with pegylated interferon and ribavirin. The level of serum HCV RNA was measured with real-time quantitative reverse-transcription polymerase chain reaction and HCV genotypes were determined by core-envelope1 region nucleotide sequencing followed by phylogenic analysis. Results There were five different subtypes of HCV in 113 patients，including 1b（40.7%），2a（13.3%），3a（9.7%），3b（17.7%）and 6a（18.6%）. The serum HCV RNA（lgcopies/ml）of them were 6.6973±1.19245，6.0824±1.68603，6.8393±1.03400，6.5533±1.29577 and 7.1503±1.16115，respectively；There was no significant relationship between serum HCV RNA and genotypes（F=1.652，P=0.166）；The rate of SVR in patients with higher serum HCV RNA（≥107copies/ml） was similar to that seen in patients with lower serum HCV RNA（＜107copies/ml），whether in the total patients（76.4%vs.75.9%，P=0.950），or in the HCV1b infections（66.7% vs.60.0%，P=0.641）or in the non-HCV1 infected patients（82.4%vs.87.9%，P=0.526）. Conclusions The levels of HCV RNA differ neither in different subtypes of HCV infection nor in different responses to pegylated interferon and ribavirin therapy.

Objective To investigate the clinical characteristics of patients with liver cirrhosis complicated by upper gastrointestinal hemorrhage（UGH）and acute cerebral infarction（ACI）. Methods The patients with UGH and ACI were compared with those without. Results The risk factors included the amount of gastrointestinal bleeding（x2=24.238，P<0.01），lienectomy（x2=37.10，P<0.01），Child-Pugh scores and ascites（x2=29.002，P<0.01），arteriosclerosis and use of antifibrinolytic agent.Conclusions For the patients with liver cirrhosis，the risk factors for ACI should be taken into consideration to prevent its occurrence.

Objective To analyze the clinical features of hepatogenous diabetes（HD）in patients with liver cirrhosis. Methods Clinical data of 45 patients with HD arising from liver cirrhosis were analyzed retrospectively. Results Of the 45 cases，there were 31 patients（68.9%）with hepatitis B virus（HBV）-related liver cirrhosis.The fasting plasma glucose（FPG）level was 7.3±2.9mmol/L and the 2-hour postprandial plasma glucose（PPG） level was 16.2±4.7mmol/L. FPG level was positively correlated with Child-Pugh score（rs=0.48，P＜0.01）. Most patients showed normal or nearly normal plasma glucose levels after they received the treatment with diet controls combined with alpha-glucosidase inhibitors or insulin. 7 patients died of the complications of liver cirrhosis. Conclusion The symptoms of diabetes mellitus in patients with HD are atypical. HD is characterized by high PPG level. Plasma glucose level correlates strongly with liver dysfunction. Insulin treatment is effective for most patients with HD. Poor prognosis is closely related to liver cirrhosis.

Objective To explore the risk factors of spontaneous bacterial peritonitis in patients with liver cirrhosis. Methods 398 patients with liver cirrhosis were divided into spontaneous bacterial peritonitis group（n=135）and non-spontaneous bacterial peritonitis group（n=263）. The predisposing factors in the two groups were compared. Results There were significant differences in history of SBP，ascites period，diabetes mellitus，total protein in ascitic fluid，digestive tract hemorrhage，Child-Pugh grades，antibiotic prophylaxis，blood Na+，serum TBil，ALB and PT between the two groups（P<0.01）. Conclusions The incidences of SBP is related to some factors，which might be properly managed in order to reduce the occurrence of spontaneous bacterial peritonitis in patients with liver cirrhosis.

Objective To evaluate the clinical significance of serum retinol-binding protein 4（RBP4）in the diagnosis of patients with nonalcoholic steatohepatitis（NASH）. Methods Serum RBP4 was measured in triplicates by a specific enzyme-linked immunosorbent assay in 42 biopsy-proven patients with NASH，28 with fatty liver and 20 healthy people. The diagnostic performance of the serum RBP4 was assessed by analysis of receiver operating characteristic（ROC）curve. Results Serum RBP4 level in the NASH was 37.11±7.68mg/L，significantly higher than that in the simple steatosis cases（22.83±6.33mg/L，P<0.01）or in control subjects（19.38±5.46mg/L，P<0.01）；The area under the curve for serum RBP4 were 0.804 and the sensitivity and specificity for diagnosis of NASH were 79.6%和76.6%，respectively. Conclusion This study demonstrates that serum RBP4 levels may be a sensitive marker to detect patients with NASH.

Objective To investigate the clinical significance of serum bilirubin and thyroid hormone levels in patients with diabetes mellitus type 2（T2DM）. Methods Blood from 87 patients with T2DM and 45 normal persons were tested the level of serum bilirubin and thyroid hormones. Results The FT3 and IBIL levels in T2DM were 2.6±1.1pmol/L and 5.0±2. 8μmol/L，and in normal control group were 4.8±1.0pmol/L and 8.8±2.6μmol/L，respectively;the FT3 levels in the poor glycemic control group，bad glycemic control group and normal control group were 3.88±0.67 pmol/L，3.16±0.84 pmol/L and 4.38±0.51pmol/L，and the IBIL levels were 7.25±4.38μmol/L，5.93±2.97μmol/L and 8.56±3.32μmol/L，respectively；The serum IBIL and FT3 levels in mild diabetes groups were 3.03±1.21pmol/L and 7.51±3.17μmol/L，in severe groups were 1.91±0.86pmol/L and 6.25±2.85μmol/L；the serum IBIL and FT3 levels in severe groups after treatment were 3.55±1.29pmol/L and 8.11±3.06μmol/L. Conclusion The changes of FT3 and IBIL levels are closely related to the progress and curative effect in T2DM.

Objective To summarize nursing organization and management in outbreak of infectious diseases in military personnel. Methods From December 2007 to January 2008，a paroxysmal episode of acute respiratory infections in military personnel occurred in Hefei. We started up the emergent response in handling it，including treating patients，taking prophylaxis for close-contacted individuals，and preventing medical staff from infection. Results A total of 108 patients with acute respiratory infections were successfully cured，and no secondary infection occurred. Conclusion The reasonable and efficient nursing management is important in dealing with abrupt communicable disease in military camp.