Abstract: Objective To investigate the effect of boschniakia rossica ethanol extract（BREE） on hydroxyproline（Hyp） and inflammatory cytokines in rats with non-alcoholic fatty liver diseases （NAFLD）. Methods The model of NAFLD in rats was established by hight-fat diet administration. Rats were randomly divided into control,model and BREE treatment group （500 mg·kg^-1·d^-1）. All rats were sacrificed to harvest serum and liver tissues at the end of eighth week. Serum levels of AST,ALT and reduced glutathione （GSH） were measured,and the levels of interleukin （IL）-1β,tumor necrosis factor（TNF）-α and IL-6 in liver tissues were detected by ELSIA. HE staining and immunohistochemistry were performed for evaluation of liver pathological changes and the expression of NF-kB/p65 in liver tissues,respectively. Results Serum levels of ALT [（39.47±20.26） U/L] and AST[（46.48 ± 18.52） U/L] in rats subjected to BREE treatment significantly decreased as compared to in the model group [（79.32±19.05）U/L and （88.35±17.46） U/L,respectively,P<0.01];Serum GSH levels in BREE-treated rats [（349.43±45.52） mg/L] significantly increased than in the model [（265.38±28.57） mg/L,P<0.01];The positive rates of NF-kB/p65 [（15.49±4.78）%] and Hyp contents [（14.28±3.08） mg/g] in liver tissues in BREE-treated rats significantly decreased than in the model [（87.54±6.59）% and （35.47±4.53）mg/g,respectively,P<0.01];the IL-1β,TNF-α and IL-6 levels[（34.51±5.61） pg/mL,（45.37±7.03） pg/mL,（18.98±5.04） pg/mL,respectively] in liver tissues in BREE-treated rats were significantly lower than in the model [（78.25±6.51） pg/mL,（85.64±6.25） pg/mL,（29.19±4.82） pg/mL,respectively,P<0.01];BREE also significantly alleviated pathological changes in the liver. Conclusions BREE significantly improves liver function and collagen deposition in rats with NAFLD by down regulation of NF-κB/p65 and inflammatory cytokines in the liver.

Key words: Non-alcoholic fatty liver disease, Boschniakia rossica ethanol extract, Nuclear transcription factor κ, B/p65, Inflammatory cytokines