Abstract: Objective To explore the dynamic changes of cytochrome P4502El（CYP2E1）in liver tissues of rats with hepatic stress injury（HSI） secondary to traumatic brain injury（TBI）. Methods Forty healthy male Wistar rats were randomly divided into sham-operated and TBI group. The animal model was established by an improved Feeney method. Serum levels of ALT and AST were measured by enzymatic assay at 6,12 and 24 h after TBI;MDA contents in liver tissues were also measured. Pathological changes of liver tissues were observed under light microscopy. The CYP2E1 mRNA levels and its protein expression were detected by RT-PCR and Western blot,respectively. Results At 6 h and 24 h after TBI,serum ALT elevated significantly [（83.0±10.3） U/L and（1204.5±146.6） U/L,respectively,P<0.01）] as compared with baseline [（42.2±8.1） U/L];at 12 h after TBI,serum AST elevated significantly[（280.4±53.3） U/L,P<0.01）] compared with baseline[（44.0±7.2）） U/L],and it peaked at 24 h[（790.3±114.5） U/L];at 6 h after TBI,serum MDA elevated significantly [（14.2±0.2） nmol/mg,P<0.05] compared with baseline[（5.2±0.2） nmol/mg],and it peaked at 24 h after TBI [（56.3±0.5）nmol/mg];at 24 h after TBI,the injuries in liver tissues were serious,with expanded sinus,scattered spotty necrosis and inflammatory cell infiltration;at 6 h after TBI,both mRNA and protein levels of CYP2E1 were significantly elevated[（0.89±0.05）and（120.24±1.22）,P<0.05] compared with baseline[（0.28±0.02） and（61.68±0.60）,respectively],and they peaked at 24 h after TBI[（1.50±0.02）and（200.40±2.61）,respectively,P<0.01]. ConclusionsCYP2E1 may be involved in oxidative stress in hepatic stress injury after TBI.

Key words: Hepatic stress injury, Cytochrome P4502El, Oxidative stress, Rats