Abstract: Objectiv To observe the inhibitory effect of 1,25（OH）₂D₃ on liver fibrosis in hepatic stellate cells （HSCs） and in rat model. Method The CCl₄-induced liver fibrosis was made in rats,and the activation of HSCs were induced by 10 pmmol/L TGF-β1 incubation. The miR-146a mimic/inhibitor were transfected in HSCs and 1,25（OH）₂D₃ intervention were paralleled. The miR-146a levels in liver tissues were detected by qPCR,the proliferation of cells was measured by CCK8 and the apoptosis was detected by flow cytometry. Results At the end of 8 weeks,the hepatic fibrosis in 1,25（OH）₂D₃ intervention group was significantly milder than that in the control group;the miR-146a level in 1,25（OH）₂D₃ intervention group was significantly higher than in oil control group[（0.70±0.03） vs.（0.33±0.17）,P<0.05];the proliferation rate in1,25（OH）₂D₃ group was 58.8%,15.91% of lower than in DMSO group,in miR-146a mimic transfected group was 46.5%,53.3% of lower than in control,in miR-146a inhibitor group was132.8%,32.8% of higher than in control（P<0.05）;the apoptosis rate of HSCs in1,25（OH）₂D₃ group was 12.6%,5.2% of higher than in DMSO,in miR-146a mimic transfected group was16.8%,8.2% of higher than in control,and in miR-146a inhibitor transfected group was 6.3%,2.2% of lower than in control （P<0.05）,indicating the inhibition of 1,25（OH）₂D₃ on proliferation and promotion on apoptosis of HSCs. Conclusion 1,25（OH）2D3 might modulate miR-146a levels to inhibit liver fibrosis.

Key words: Liver fibrosis, Hepatic stellate cells, 1, 25（OH）₂D₃, miR-146a, Rats