1,25（OH）2D3通过STAT5抑制Th17细胞分化的调控作用研究*

doi:10.3969/j.issn.1672-5069.2016.04.006

实用肝脏病杂志 ›› 2016, Vol. 19 ›› Issue (4): 404-407.doi: 10.3969/j.issn.1672-5069.2016.04.006

1,25（OH）2D3通过STAT5抑制Th17细胞分化的调控作用研究^*

周鸿, 顾磊, 蒋春晖, 孙隆慈, 刘晔, 许春杰, 徐庆

200127上海市交通大学医学院附属仁济医院胃肠外科

收稿日期:2016-01-10 出版日期:2016-07-30 发布日期:2016-08-31
通讯作者: 徐庆,E-mail:renjixuqing@163.com
作者简介:周鸿,男,41岁,博士研究生,主治医师。主要从事消化内镜诊断与治疗学研究。E-mail:renjizhouhong@163.com
基金资助:
上海市卫生和计划生育委员会科研课题（编号：20154Y0207）

1,25-（OH）₂D₃ inhibits Th17 cell differentiation via STAT5 signaling

Zhou Hong, Gu Lei, Jiang Chunhui, et al

Department of Gastrointestinal Surgery,Renji Hospital,School of Medicine,JiaoTong University,Shanghai 200127,China

Received:2016-01-10 Online:2016-07-30 Published:2016-08-31

摘要/Abstract

摘要： 目的研究1.25二羟基维生素D₃[1,25（OH）₂D₃]抑制辅助性T细胞17（Th17）的分化与STAT5调控的关系。方法通过分选出的CD4⁺T细胞,在1,25（OH）₂D₃和/或STAT5抑制剂的作用下,采用ELISA法检测抑制剂处理后细胞培养上清液Th17细胞因子（IL-17A、IL-22）水平的变化;采用细胞免疫荧光技术检测STAT5的磷酸化水平,采用Western blot技术检测STAT5蛋白表达水平。结果 1,25（OH）₂D₃组细胞培养上清IL-17A和IL-22水平（12.5±0.5 ng/ml,48.5±0.9 pg/ml）明显低于对照组（22.7±0.5 ng/ml,73.8±1.9 pg/ml）,而STAT5抑制剂组IL-17A和IL-22水平明显升高（33.5±0.7 ng/ml,89.1±1.4 pg/ml）,1,25（OH）₂D₃与STAT5抑制剂联合作用细胞IL-17A和IL-22水平（18.5±0.7 ng/ml,54.1±1.6 pg/ml）显著高于1,25（OH）₂D₃组,但低于STAT5抑制剂组,差异有统计学意义（P<0.01）;1,25（OH）₂D₃组细胞p-STAT5表达显著强于对照组,1,25（OH）₂D₃联合STAT5抑制剂组p-STAT5表达量低于对照组,而STAT5抑制剂组细胞p-STAT5表达量最低;1,25（OH）₂D₃组STAT5蛋白表达明显升高,而1,25（OH）₂D₃联合STAT5抑制剂组或STAT5抑制剂组STAT5蛋白表达明显降低,以STAT5抑制剂组细胞表达最弱,差异均具有统计学意义（P<0.01）。结论 1,25（OH）₂D₃通过STAT5信号通路能抑制Th17细胞分化。

关键词: 辅助性T 细胞17, 1,25-二羟基维生素D₃, STAT5, 细胞分化, 小鼠

Abstract: Objective To explore the role of 1,25-（OH）₂D₃ in inhibiting T helper cell 17（Th17） differentiation and the role of STAT5 in this process. Methods Sorted CD4⁺T cells were treated with 1,25-（OH）₂D₃ and/or STAT5 inhibitors. The Th17 cytokines （IL-17A,IL-22） levels in supernatants were detected by ELISA;the phosphorylation level of STAT5 was examined by cell immunofluorescence,and Western blot was used to detect STAT5 protein expression. Results The levels of IL-17A and IL-22 in 1,25-（OH）₂D₃-treated group were significantly lower than those in the control group [（12.5±0.5） ng/ml and （22.7±0.5） pg/ml vs. （48.5±0.9） ng/ml and（73.8±1.9） pg/ml,respectively,P<0.01],while in STAT5 inhibitor-treated group had higher levels of IL-17A [（33.5±0.7） ng/ml] and IL-22 [（89.1±1.4） pg/ml,P<0.05];IL-17A [（18.5±0.7） ng/ml] and IL-22 [（54.1±1.6） pg/ml] levels in cells treated with 1,25（OH）₂D₃ combined with STAT5 inhibitor were significantly higher than those in 1,25-（OH）₂D₃-treated alone,whereas lower than those in STAT5 inhibitor-treated cells （P<0.01 for both）;the expression of p-STAT5 was higher in 1,25-（OH）₂D₃-treated cells and lower in 1,25-（OH）₂D₃ combined with STAT5 inhibitor-treated cells,while in STAT5 inhibitor-treated cells had the lowest p-STAT5; the STAT5 protein expression was significantly higher in 1,25-（OH）₂D₃-treated cells,whereas lower in 1,25-（OH）₂D₃ combined with STAT5 inhibitor-treated cells or in STAT5 inhibitor-treated cells had the lowest STAT5 protein expression （P<0.01 for all）. Conclusion 1,25-（OH）₂D₃ might inhibit Th17 cell differentiation via STAT5 signaling pathway.

Key words: T helper cell 17, 1,25-（OH）₂D₃, STAT5, Cell differentiation, Mice

周鸿, 顾磊, 蒋春晖, 孙隆慈, 刘晔, 许春杰, 徐庆. 1,25（OH）2D3通过STAT5抑制Th17细胞分化的调控作用研究^*[J]. 实用肝脏病杂志, 2016, 19(4): 404-407.

Zhou Hong, Gu Lei, Jiang Chunhui, et al. 1,25-（OH）₂D₃ inhibits Th17 cell differentiation via STAT5 signaling[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2016, 19(4): 404-407.

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1,25（OH）2D3通过STAT5抑制Th17细胞分化的调控作用研究^*

1,25-（OH）₂D₃ inhibits Th17 cell differentiation via STAT5 signaling

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1,25（OH）2D3通过STAT5抑制Th17细胞分化的调控作用研究*

1,25-（OH）2D3 inhibits Th17 cell differentiation via STAT5 signaling

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1,25（OH）2D3通过STAT5抑制Th17细胞分化的调控作用研究^*

1,25-（OH）₂D₃ inhibits Th17 cell differentiation via STAT5 signaling