对乙酰氨基酚诱导的急性肝损伤小鼠肝组织糖基转移酶Colgalt2表达的变化*

doi:10.3969/j.issn.1672-5069.2019.01.009

实用肝脏病杂志 ›› 2019, Vol. 22 ›› Issue (1): 29-32.doi: 10.3969/j.issn.1672-5069.2019.01.009

对乙酰氨基酚诱导的急性肝损伤小鼠肝组织糖基转移酶Colgalt2表达的变化^*

郭乐乐, 张晓慧, 张向颖, 胡中杰, 任锋, 张晶

100069 北京市首都医科大学附属北京佑安医院丙型肝炎与中毒性肝病科（郭乐乐,张晓慧,张晶,胡中杰）; 北京市肝病研究所（任锋,张向颖）

收稿日期:2017-12-20 出版日期:2019-01-10 发布日期:2019-01-16
通讯作者: 张晶,E-mail:drzhangjing@163.com
作者简介:郭乐乐,女,28岁,硕士研究生。主要从事急性药物性肝损伤和糖基转移酶Colgalt2研究。E-mali:Glele1123@163.com
基金资助:
*国家自然科学基金项目（编号：81770611/81270532）; 北京市自然科学基金项目（编号：7162085）; 首都特色临床应用研究项目（编号：Z121107001012167）; 北京市卫生系统高层次卫生技术人才培养计划项目（编号：2013-3-075）

Expression of glycosyltransferase Colgalt2 in liver tissues of mice with acetaminophen-induced acute liver injury

Guo Lele, Zhang Xiaohui, Zhang Xiangying, et al.

Department of Hepatitis C & Toxic Liver Diseases,You’an Hospital,Capital Medical University,Beijing 100069,China

Received:2017-12-20 Online:2019-01-10 Published:2019-01-16

摘要/Abstract

摘要： 目的探讨对乙酰氨基酚（APAP）诱导的急性肝损伤小鼠肝组织糖基转移酶Colgalt2表达的变化。方法取C57BL/6小鼠100只,采用腹腔注射APAP溶液法建立小鼠急性肝损伤模型。首先建立不同浓度干预组,每组10只,分别给予【0 mg·kg^-1 APAP（对照组）、100 mg·kg^-1 APAP处理组、250 mg.kg^-1 APAP处理组、500 mg·kg^-1 APAP处理组和1000 mg·kg^-1 APAP处理组】,再建立不同时间干预组,每组10只,均给予500 mg·kg^-1 APAP腹腔注射,分别观察【0 h APAP（对照组）、2 h APAP处理组、4 h APAP处理组、8 h APAP处理组和12 h APAP处理组】。采用Real-time quantitative PCR 和Western blot法检测肝组织对糖基转移酶Colgalt2 基因及其蛋白表达情况。结果在250 mg·kg^-1、500 mg·kg^-1和1000 mg·kg^-1 APAP处理组,血清ALT分别为（1360.5±189.8）IU/L、（3191.2±118.6）IU/L和（5022.7±234.6）IU/L,AST分别为（2147.1±133.4）IU/L、（3628.8±107.9）IU/L和（5854.5±295.1）IU/L,较对照组显著升高（P<0.05）;在4 h、8 h和12 h APAP处理组,血清ALT水平有类似的变化;在100 mg·kg^-1、250 mg·kg^-1、500 mg·kg^-1、1000 mg·kg^-1 APAP处理组,肝组织Colgalt2基因水平较对照组显著升高;在2 h、4 h、8 h和12 h APAP处理组,肝组织Colgalt2基因水平较对照组逐渐显著升高,肝组织Colgalt2蛋白表达水平也随APAP干预剂量的增加和干预时间的延长也呈逐渐增强趋势。结论肝组织糖基转移酶Colgalt2在对乙酰氨基酚诱导的急性肝损伤小鼠发病过程中发挥着重要的作用。

关键词: 急性肝损伤, 对乙酰氨基酚, 糖基转移酶, Colgalt2, 小鼠

Abstract: Objective To investigate the expression of glycosyltransferase Colgalt2 in liver tissues of mice with acetaminophen （APAP）-induced acute liver injury （ALI）. Methods One hundred of C57BL/6 mice were included,and the model of ALI was established by intraperitoneal injection of APAP at 0 mg·kg^-1 APAP（control）,100 mg·kg^-1,250 mg·kg^-1,500mg·kg^-1 and 1000mg·kg^-1 group （APAP concentration model,10 in each） or by intraperitoneal injection of APAP at 500 mg·kg^-1 for 0 h（control）,2 h,4 h,8 h and 12 h group（time model）. Hepatic Colgalt2 mRNA and its protein were detected by real-time PCR and Western blot,respectively. Results Serum ALT levels were（1360.5±189.8） IU/L,（3191.2±118.6） IU/L and （5022.7±234.6） IU/L,and serum AST levels were （2147.1±133.4） IU/L,（3628.8±107.9） IU/L and（5854.5±295.1） IU/L in 250 mg.kg^-1,500 mg·kg^-1 and 1000 mg·kg^-1 APAP-intervened groups,significantly increased as compared to those in the control（P<0.05）;serum ALT levels were（694.1±63.5） IU/L,（1321.9±83.5） IU/L and（3151.1±162.7） IU/L,and serum AST levels were（857.5±69.2） IU/L,（1658.5±109.5） IU/L and（3395.2±620.6） IU/L,respectively,at 4 h,8 h and 12 h APAP group,significantly increased as compared to those in the control（P<0.05）;the hepatic Colgalt2 mRNA levels significantly increased at 500 mg·kg^-1 and 1000 mg·kg^-1 APAP groups,and they also gradually increased at 2 h,4 h,8 h and 12 h APAP group as compared to those in the control（P<0.05）,and the expression of Colgalt2 protein was similar gradually increased as its gene did. Conclusion Glycosyltransferase Colgalt2 plays an important role in the pathogenesis of mice with acetaminophen-induced ALI.

Key words: Acute liver injury, Acetaminophen, Glycosyltransferase, Colgalt2, Mice

郭乐乐, 张晓慧, 张向颖, 胡中杰, 任锋, 张晶. 对乙酰氨基酚诱导的急性肝损伤小鼠肝组织糖基转移酶Colgalt2表达的变化^*[J]. 实用肝脏病杂志, 2019, 22(1): 29-32.

Guo Lele, Zhang Xiaohui, Zhang Xiangying, et al.. Expression of glycosyltransferase Colgalt2 in liver tissues of mice with acetaminophen-induced acute liver injury[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2019, 22(1): 29-32.

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对乙酰氨基酚诱导的急性肝损伤小鼠肝组织糖基转移酶Colgalt2表达的变化^*

Expression of glycosyltransferase Colgalt2 in liver tissues of mice with acetaminophen-induced acute liver injury

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