关键词: 肝细胞癌, miR-144脂质体, HepG2, SMMC-7721细胞, 裸鼠

Abstract: Objective To observe the inhibition of miR-144 liposomes on proliferation,migration and invasion of liver cancer cells in vitro and in vivo. Methods DOTMA cationic liposomes were prepared by thin film dispersion method,and miR-144 liposomes were prepared by incubating liposome with miR-144. The volume to mass ratio of DOTMP liposomes and miR-144 were determined by uptake and transfection experiments. The cytotoxicity of liposome on HepG2 and SMMC-7721 cells,impacts on cell migration and invasiveness and the inhibitory effect on implanted tumor sizes in nude mice were observed. Results When the volume mass ratio of DOTMP liposomes and miR-144 was 3:1,the particle size of miR-144 liposome complex was about 200 nm with the PDI of less than 0.3;the transfection efficiency of DOTMP liposomes was the highest when the volume/mass ratio of plasmids and miR-144（mu l/g） was 3:1（P<0.05）;the HepG2 and SMMC-7721 cells intook much more miR-144 liposome complex as incubation prolonged（P<0.05）;the migration distance of HepG2 and SMMC-7721 cells were both significantly shorten by miR-144 liposome complex as compared with in the control group（P<0.05）;the volumes of implanted miR-144 liposome complex intervened HepG2 and SMMC-7721 cell tumors were significantly smaller than in the control group （P<0.05）. Conclusion MiR-144 liposomes might inhibit the activity of hepatocellular carcinoma cells in vitro and in vivo,which worth further investigation.

Key words: Hepatoma, miR-144 liposomes, HepG2, SMMC-7721 cells, Nude mice