关键词: 肝细胞癌, 微小RNA, 病理学, 预后

Abstract: Objective To investigate the relationship between cancerous miRNA-30c levels and the survival of patients with hepatocellular caicinoma （HCC） after hepatectomy. Methods Thirty-five patients with HCC in our hospital between February 2010 and November 2012 were included in this study. All patients were confirmed by pathological examination after hepatectomy,and cancerous miRNA-30c levels were assayed by real-time quantitative polymerase chain reaction. Cancerous miRNA-30c levels were compared between patients with different clinical and pathological features. The survival rate of patients was evaluated by the Kaplan-Meier minimum product method,and the difference of survival rate was evaluated by Log-Rank test. Results The relative level of cancerous miRNA-30c in the 35 patients with HCC was 0.6, with 95% CI of 0.3 to 0.6,and there were 13 patientss with low cancerous miRNA-30c level less than 0.6 and 22 patients with high cancerous miRNA-30c levels greater than 0.6;there were no significant differences of cancerous miRNA-30c levels between patients with diffenrent gender,age,tumor size,degree of differentiation,TNM staging,history of viral hepatitis,and serum AFP levels（P>0.05）,however,the cancerous miRNA-30c levels in 17 patients with lymph node metastasis was（0.2±0.0）,significantly lower than 【（0.8±0.1）,P<0.05】 in 18 patients without lymph node matastasis;the adjusted risk ratio （OR）=5.4,and 95% CI:1.2 to 20.1;the total survival in patients with high cancerous miRNA-30c levels was （14.5±6.7） m,with 95%CI of 11.0-28.4 m,significantly longer than【（7.9±1.5） m,with 95%CI of 3.2-10.7 m,P<0.05】 in patients with lower cancerous miRNA-30c levels. Conclusion Our findings indicate that the cancerous miRNA-30c level is related to the prognosis of patients with hepatocellular carcinoma,and it might be a new potential biomarker for predicting the survival of patients with hepatocellular carcinoma after hepatectomy.

Key words: Hepatocellular carcinoma, micro RNA, Pathology, Prognosis