实用肝脏病杂志 ›› 2019, Vol. 22 ›› Issue (4): 486-489.doi: 10.3969/j.issn.1672-5069.2019.04.009

• 实验性肝炎 • 上一篇    下一篇

去甲斑蝥酸钠对肝癌异位移植瘤小鼠肠道微生态的影响初探*

裴颖, 黄峰, 殷宏振, 郭化磊   

  1. 712000 陕西省咸阳市陕西中医药大学第一临床医学院
  • 收稿日期:2018-09-13 出版日期:2019-07-10 发布日期:2019-07-19
  • 通讯作者: 黄峰;E-mail:hf9939@163.com
  • 作者简介:裴颖,男,25 岁,硕士研究生。研究方向:肝癌与肠道微生态。E-mail:1098917826@qq.com
  • 基金资助:
    * 陕西省自然科学基础研究计划项目(编号:2014JM4175)

Preliminary study on intestinal microecology in mice with xenograft hepatoma intervened by sodiumnorcantharidate

Pei Ying, Huang Feng, Yin Hongzhen, et al   

  1. First Clinical Medical College,Shaanxi University of Traditional Chinese Medicine,Xianyang 712000,Shaanxi Province,China
  • Received:2018-09-13 Online:2019-07-10 Published:2019-07-19

摘要: 目的 初步探讨去甲斑蝥酸钠对肝癌异位移植瘤小鼠肠道微生态的影响。方法 将12 只小鼠随机分为去甲斑蝥酸钠注射液和生理盐水处理组及对照组,建立H22 肝癌异位移植瘤小鼠模型。造模成功后正常饲养动物2 w,然后采集小鼠粪便,并进行Illumina miseq 2×300 bp 高通量测序及生物信息学分析。结果 各组样本所包含物种量丰富,且物种丰度和均匀度趋于一致;去甲斑蝥酸钠注射液组与生理盐水处理组或对照组比,存在显著的菌群差异;在门水平上,差异最显著的菌群为变形菌门,去甲斑蝥酸钠注射液组变形菌门所占比例为9.1%,显著高于生理盐水处理组或对照组(分别为2.8%和2.3%,P<0.05);在属水平上,差异显著的菌群包括拟杆菌属等,生理盐水处理组拟杆菌属所占比例为26.8%,显著高于去甲斑蝥酸钠注射液组或对照组(分别为13.2%和6.8%,P<0.05)。结论 去甲斑蝥酸钠对肝癌异位移植瘤小鼠肠道微生态有显著的影响,或许有助于抗肿瘤作用。

关键词: 肝癌, 去甲斑蝥酸钠, 肠道微生态, 小鼠

Abstract: Objective To explore the changes of intestinal microecology in mice with xenograft hepatoma intervened by sodium norcantharidate.Methods Twelve Kunming mice were randomly divided into sodium nor-cantharidate-intervend,saline-intervend and control groups,and a mouse model of H22 hepatoma xenograft was es-tablished in sodium norcantharidate and saline groups. The mice were normally fed for 2 weeks after the success-ful establishment of the model. Then,the fecal samples were collected and Illumina miseq 2×300 bp high -throughput sequencing and bioinformatics analysis were performed.Results Every samples in each group con-tained abundant species,and species abundance and evenness tended to be consistent;there were significant differ-ences as respect to bacterial communities among sodium norcantharidate,saline and control group;at phylum,the most significantly different bacterial community was Proteobacteria and in sodium norcantharidate-intervend group,the proportion of Proteobacteria was 9.1%,significantly higher than those in saline group or the control group(2.8% and 2.3% ,respectively,P <0.05);in genus,the different bacterial community included Bacteroides,etc;in saline-intervened group,the proportion of Bacteroides was 26.8%,significantly higher than those in sodium norcan-tharidate group or the control group (13.2% and 6.8%,respectively,P<0.05). Conclusion There is a obvious change in intestinal microecology in mice with hepatoma xenograft receiving sodium norcantharidate intervention,which might be related to the anti-tumor effect of this agent.

Key words: Hepatoma, Sodium norcantharidate, Intestinal microecology, Mice