抗增殖蛋白过表达对HepG2细胞增殖和凋亡的影响*

doi:10.3969/j.issn.1672-5069.2016.04.007

实用肝脏病杂志 ›› 2016, Vol. 19 ›› Issue (4): 408-412.doi: 10.3969/j.issn.1672-5069.2016.04.007

抗增殖蛋白过表达对HepG₂细胞增殖和凋亡的影响^*

翟嵩, 孙明珠, 王文俊, 李亚萍, 张欣, 李梅, 党双锁

710004 西安市西安交通大学医学院第二附属医院感染病科

收稿日期:2016-01-04 出版日期:2016-07-30 发布日期:2016-08-31
通讯作者: 党双锁,E-mail:dang212@126.com
作者简介:翟嵩,女,38岁,医学博士,主治医师。主要从事感染性疾病发病机制研究。E-mail:zhaisongsong@189.cn
基金资助:
国家自然科学基金资助项目（81170393）

Effect of prohibitin over-expression on proliferation and apoptosis of human hepatocellular carcinoma cell line HepG2 cells

Zhai Song, Sun Mingzhu, Wang Wenjun, et al

Department of Infectious Diseases,Second Affiliated Hospital,Medical School,Jiaotong University,Xi’an 710004,Shaanxi Province,China

Received:2016-01-04 Online:2016-07-30 Published:2016-08-31

摘要/Abstract

摘要： 目的探讨抗增殖蛋白（PHB）过表达对人肝癌细胞系HepG₂细胞增殖和凋亡的影响。方法在已构建的PHB真核表达质粒pEGFP-N1-PHB瞬时转染HepG₂细胞,采用四甲基偶氮唑盐比色法（MTT法）检测HepG₂细胞增殖;使用流式细胞仪检测HepG₂细胞周期和凋亡。结果转染pEGFP-N1-PHB细胞增殖明显低于pEGFP-N1空载转染细胞或未转染细胞（P<0.05）;在转染后48 h,转染组G2/M期细胞比例为（27.84±0.47）%,显著高于空载转染组的（17.21±0.64）%或未转染组的（22.67±0.33）%（P<0.001）;转染组细胞凋亡率为（31.72±0.35）%,显著高于空载转染组的（18.66±0.56）%或未转染组的（13.47±0.94）%（P<0.001）。结论 PHB过表达可抑制人肝癌HepG₂细胞增殖,诱导细胞进入G2/M期后被阻滞,并诱导细胞凋亡。

关键词: HepG₂细胞, 抗增殖蛋白, 细胞凋亡, 细胞增殖

Abstract: Objective To investigate the effect of prohibitin（PHB） over-expression on proliferation and apoptosis of human hepatocellular carcinoma cell line HepG2 cells. Methods The eukaryotic expression plasmid （pEGFP-N1-PHB） had been constructed and the HepG2 cells were transiently transfected with recombinant plasmid. Measurement of cell proliferation was performed by using MTT assay. Flow cytometry was carried out to detect cell cycle alterations and apoptosis. Results The cell growth curves showed that the proliferation of HepG2 cells were significantly decreased after transfection with pEGFP-N1-PHB as compared with pEGFP-N1 or controls（P<0.05）;At 48 hours after transfection,the proportion of G2/M phase of HepG2 cells in pEGFP-N1-PHB-transfected group was（27.84±0.47）%,significantly higher than in pEGFP-N1-transfected group[（17.21± 0.64）%] or in the non-transfected group[（22.67±0.33）%,P<0.001];The percentage of apoptotic cells in pEGFP-N1-PHB-transfected group was（31.72±0.35）%,much higher than（18.66±0.56） % in pEGFP-N1-transfected or non-transfected cells [（13.47±0.94）%,P<0.001]. Conclusion PHB overexpression might inhibit the proliferation, induce G2/M phase arrest and apoptosis of HepG2 cells.

Key words: Hep G₂ Cells, Prohibitin, Apoptosis, Cell cycle

翟嵩, 孙明珠, 王文俊, 李亚萍, 张欣, 李梅, 党双锁. 抗增殖蛋白过表达对HepG₂细胞增殖和凋亡的影响^*[J]. 实用肝脏病杂志, 2016, 19(4): 408-412.

Zhai Song, Sun Mingzhu, Wang Wenjun, et al. Effect of prohibitin over-expression on proliferation and apoptosis of human hepatocellular carcinoma cell line HepG2 cells[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2016, 19(4): 408-412.

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抗增殖蛋白过表达对HepG₂细胞增殖和凋亡的影响^*

Effect of prohibitin over-expression on proliferation and apoptosis of human hepatocellular carcinoma cell line HepG2 cells

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