Abstract: Objective To investigate the efficacy of bacillus subtilis duplex and polyene phosphatidylcholine combination in the treatment of patients with nonalcoholic fatty liver diseases （NAFLD） and its effects on serum people end limb homologous protein 2（Pygo2）,high mobility group protein B1（HMGB1） and hypoxia inducible factor-1α （HIF-1α） levels. Methods 78 patients with NAFLD admitted to our hospital between June 2015 and June 2016 were divided randomly into observation（n=39） and control group （n=39）,and received bacillus subtilis duplex and polyene phosphatidylcholine combination or the latter alone for three months. Serum Pygo2,HMGB1 and HIF-1α were detected by using ELISA. Results At the end of three month treatment,serum levels of TG, TC,LDL-C and HDL-C in the observation group were（1.2±0.2） mmol/L,（4.1±0.2） mmol/L,（3.1±0.1） mmol/L and （3.9±0.3） mmol/L,significantly different compared to[（1.6±0.3） mmol/L,（5.1±0.3） mmol/L,（3.5±0.2） mmol/L and （3.2±0.2） mmol/L in the control,P<0.05];serum levels of MDA and SOD were （4.4±0.7） μmol/L and （168.9±15.7） U/L,significantly different compared to[（5.4±0.8） μmol/L and（154.4±15.3） U/L in the control,P<0.05]; serum levels of Pygo2,HMGB1 and HIF-1 alpha were（41.5±3.4）μg/L,（15.7±4.6） μg/L and（24.5±4.6）μg/L, respectively, significantly lower than [（48.2±3.7） μg/L,（26.4±5.1） μg/L and（32.6±4.7） μg/L in the control,P<0.05]; the percentages of normal liver, and mild, moderate and severe fatty liver were 56.4%,and 35.9%,5.1% and 2.6%,respectively,significantly better than（10.3%, and 30.8%,41.0% and 17.9%,P<0.05） in the control. Conclusion The application of bacillus subtilis duplex and polyene phosphatidylcholine combination in the treatment of patients with NAFLD is clinically efficient,which might effectively improve blood lipid imbalance,inhibit oxidative stress,and reduce serum Pygo2,HMGB1 and HIF-1α levels.

Key words: Non-alcoholic fatty liver diseases, Bacillus subtilis, Polyene Phosphatidylcholine, Therapy