Abstract: Objective The aim of this study was to investigate the efficacy of of combination of tenofovir and Anluo Huaxian pill,a herbal medicine,in the treatment of patients with HBeAg-negative chronic hepatitis B （CHB）. Methods A total of 68 patients with HBeAg-negative CHB were recruited in our hospital bwtween March 2015 and May 2017,and were divided randomly into two groups,with 34 patients in each. The patients in the control group were treated with tenofovir orally and those in the observation were treated with combination of tenofovir and Anluo Huaxian pill for 48 weeks. Serum interleukin（IL）-6,IL-2,and tumor necrosis factor-α（TNF-α） by enzyme-linked immunosorbent assay,and serum type III procollagen（PC-III）,hyaluronic acid （HA）,laminin （LN） and type IV collagen （IV-C） were detected by radioimmunoassay. Results At the end of 48 w treatment,serum HA,LN,IV-C,and PC-III levels in the combination group were（94.5±31.5） ng/ml,（113.2±51.6） ng/ml, （72.4±20.3） g/L and （102.4±19.6） g/L,significantly lower than [（121.6±37.5） ng/ml,（132.5±53.8） ng/ml,（94.6 ±22.1） μg/L and（134.7±21.5） g/L,respectively,P<0.05] in the control;serum IL-2 level was （173.6±28.3） ng/L, significantly higher than [（145.7±26.4） ng/L,P<0.05], while serum IL- 6 and TNF-α levels were （94.5±16.2） pg/ml and（26.6±6.8） pg/ml,significantly lower than [（112.6±17.8） pg/ml and（35.7±8.3） pg/ml,respectively,P<0.05] in the control;serum HBV DNA loss were 91.2% and 88.2% in the two groups（P<0.05） and serum ALT normalization rates in the two groups were 94.1% and 94.1%. Conclusion The administration of tenofovir and Anluo Huaxian pills is effective in the treatment of patients with HBeAg-negative CHB, which might alleviate liver fibrosis and reduce intrahepatic inflammation.

Key words: Hepatitis B, Tenofovir, Anluo Huaxian pills, herbal medicine, HBeAg-negative, Cytokines, Therapy