肝脏前体细胞移植对CCl4诱导的肝纤维化自发逆转大鼠肝组织学的影响*

doi:10.3969/j.issn.1672-5069.2018.03.015

Abstract

Abstract: Objective T his study was aimed to investigate the repair potential of hepatic fibrosis in rats with carbon tetrachloride （CCl4）-induced liver fibrosis after hepatic progenitor cell （HPC） transplantation. Methods CCl4 was intraperitoneally injected for 4 weeks,8 weeks and 12 weeks to establish the model. Immunohistochemical staining was used to detect the expression of OV6-positive HPCs in liver tissues. 14 male SD model rats were randomly divided into spontaneous reversal group （n=7） and HPCs transplantation group （n=7）. HPCs transplantation was conducted by intrasplenic injection with 5×106 HPCs/0.5 ml. Liver tissues was stained with hematoxylin-eosin and sirius red,and histopathological changes were observed. The expression of OV6 and α-smooth muscle agonists（α-SMA） in liver tissues were detected by immunohistochemical staining,and the α-SMA and tissue inhibitor of matrix metalloproteinases （TIMP）-1 were detected by Western bloting. Serum type I collagen and albumin levels were measured by ELISA. Results The number of OV6 positive cells in liver tissues reached the peak at 8 weeks after intraperitoneal injection of CCl4,and decreased at 12 weeks;the degree of fibrosis was reduced in HPCs-transplanted group,and the area of collagen was significantly decreased as compared with in the spontaneous reversal group[（3.14±0.99） vs.（5.05±0.89）,P<0.05];the relative quantification of α-SMA [（1.22±0.10） vs. （0.31±0.08）] and TIMP-1 protein [（0.43±0.09） vs.（0.25±0.032）] in spontaneous reversal group was much higher as compared with in HPCs transplantation group;serum type I collagen levels in the spontaneous reversal group was higher than in the HPCs transplantation group[（41.33±8.08） ng/ml vs.（31.12±10.08） ng/ml,P<0.05]. serum albumin levels in spontaneous reversal group was remarkably lower than that in the HPCs transplanta-tion group [（33.18±4.69） g/L vs. （48.30±2.15） g/L，P<0.05]. Conclusion Hepatic progenitor cell transplantation have the potential to accelerate the reverse of liver fibrosis and improve liver function in rats with liver fibrosis.

Key words: Liver fibrosis, Hepatic progenitor cells, Cell transplantation, Rats

Chen Hui, Ren Wanlei, Yang Aiting. Hepatic progenitor cell transplantation accelerates spontaneous reverse of hepatic fibrosis in rats with carbon tetrachloride-induced liver fibrosis[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2018, 21(3): 376-379.

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Hepatic progenitor cell transplantation accelerates spontaneous reverse of hepatic fibrosis in rats with carbon tetrachloride-induced liver fibrosis

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