Abstract: Objective To explore the effects of Yinzhihuang oral solution on steatosis in rats with methionine and choline deficient diet （MCD）-induced non-alcoholic steatohepatitis （NASH）. Methods 30 male rats were randomly divided into control,MCD,and Yinzhihuang-intervened group. The animals in control group was given a standard diet,and those in MCD and Yinzhihuang group were given MCD diet. At the end of forth week,saline or Yinzhihuang oral solution were administered intragastricly, respectively,once a day for four weeks. The liver tissues was obtained for HE and Masson staining and histological analysis was examined by NAFLD activity score （NAS） system,and the blood was analyzed for biochemical parameters. Results The body weight of rats in control and MCD group were [（487.2±6.5）g and （155.8±3.1）g,P<0.05],and the liver index were [（2.92±0.06） %and （5.09±0.19）%,P<0.05];the liver tissues in MCD group showed obvious hepatic steatosis,lobular inflammation,and ballooning of hepatocytes with mild fibrosis;the liver index in Yinzhihuang group[（4.47±0.18）%] was lower than that in MCD group（P<0.05）;the NAS scores in Yinzhihuang group and MCD group were 5.9±0.2 and 7.2±0.1（P<0.05）,and the fibrosis score were 0.3±0.2 and 0.8±0.2（P<0.05）;serum ALT levels in Yinzhihuang group and MCD group were[（68.4±6.4）U/L and（111.9±5.5）U/L,P<0.05],and AST levels were[（110.5±7.9）U/L and（170.5±10.8）U/L,P<0.05],while serum TBIL,TG,TC,LDL,HDL,and FBG had no significant difference between Yinzhihuang group and MCD group. Conclusion Yinzhihuang oral solution could improve hepatic steatosis, lobular inflammation,ballooning,and fibrosis in liver tissues,suggesting its therapeutic effects on NASH in animal models.

Key words: Non-alcoholic steatohepatitis, Yinzhihuang oral solution, Methionine and choline deficient diet, Rats