Abstract: Objective To investigate the roles of Norrin/Frizzled-4 in the interstitial remodeling of acute liver injury. Methods 32 healthy male rats were randomly divided into control（n=8） and model group （n=24）. The model of acute liver injury was established by thioacetamide （TAA） injuction. Western blot was used to detect the expression of Norrin/Frizzled-4 protein in liver tissues,and enzyme-linked immunosorbent assay was performed to measure serum CD105 and vascular endothelial growth factor （VEGF） levels. Results There were 11（45.8%） out of 24 rats died in model group during model establishment. Hematoxylin-eosin staining showed that the acute liver injury model was successfully established. Serum CD105 level was（2.18±0.05） ng/mL and VEGF was （61.48±0.39） pg/mL in control group,significantly lower than[（2.36±0.07） ng/mL and [（62.09±0.54） pg/mL,P<0.05] in TAA-treated for one week,or [2.42±0.03） ng/mL and （64.52±0.25） pg/mL,P<0.05] in TAA-treated for two week;Western blot analysis showed that the expressions of Norrin/Frizzled-4 proteins in model group were higher than in normal control group or in primary hepatic stellate cells（P<0.01）. Conclusion The expressions of Norrin/Frizzled-4 proteins increase in liver tissues of rats with acute liver injury,which might be related to the maintaing of vascular network morphology and interstitial remodeling.

Key words: Acute liver injury, Norrin/Frizzled-4 signaling pathway, Interstitial reconstruction, Thioacetamide, Rats