Abstract: Objective To investigate the expression of glycosyltransferase Colgalt2 in liver tissues of mice with acetaminophen （APAP）-induced acute liver injury （ALI）. Methods One hundred of C57BL/6 mice were included,and the model of ALI was established by intraperitoneal injection of APAP at 0 mg·kg^-1 APAP（control）,100 mg·kg^-1,250 mg·kg^-1,500mg·kg^-1 and 1000mg·kg^-1 group （APAP concentration model,10 in each） or by intraperitoneal injection of APAP at 500 mg·kg^-1 for 0 h（control）,2 h,4 h,8 h and 12 h group（time model）. Hepatic Colgalt2 mRNA and its protein were detected by real-time PCR and Western blot,respectively. Results Serum ALT levels were（1360.5±189.8） IU/L,（3191.2±118.6） IU/L and （5022.7±234.6） IU/L,and serum AST levels were （2147.1±133.4） IU/L,（3628.8±107.9） IU/L and（5854.5±295.1） IU/L in 250 mg.kg^-1,500 mg·kg^-1 and 1000 mg·kg^-1 APAP-intervened groups,significantly increased as compared to those in the control（P<0.05）;serum ALT levels were（694.1±63.5） IU/L,（1321.9±83.5） IU/L and（3151.1±162.7） IU/L,and serum AST levels were（857.5±69.2） IU/L,（1658.5±109.5） IU/L and（3395.2±620.6） IU/L,respectively,at 4 h,8 h and 12 h APAP group,significantly increased as compared to those in the control（P<0.05）;the hepatic Colgalt2 mRNA levels significantly increased at 500 mg·kg^-1 and 1000 mg·kg^-1 APAP groups,and they also gradually increased at 2 h,4 h,8 h and 12 h APAP group as compared to those in the control（P<0.05）,and the expression of Colgalt2 protein was similar gradually increased as its gene did. Conclusion Glycosyltransferase Colgalt2 plays an important role in the pathogenesis of mice with acetaminophen-induced ALI.

Key words: Acute liver injury, Acetaminophen, Glycosyltransferase, Colgalt2, Mice