Abstract: Objective To establish the acute or chronic HBV-infection mouse model. Methods Acute or chronic HBV-infection mouse model was established by hydrodynamic injection of pAAV/HBV1.2 plasmid. Serum HBsAg were detected by ELISA. The immune-related molecule mRNA were detected by real-time PCR. The activated lymphocytes in the livers were detected by FACS and ELISPOT was used to detect the HBV-specific cellular immune response. Results We successfully established the acute and chronic HBV-infection mouse model by hydrodynamic injection of pAAV/HBV1.2 plasmid；The duration of serum HBsAg secretion in acute HBV-infected BALB/c mice was （3.25±1.04） weeks，significantly shorter than that in chronic HBV-infected C57BL/6 mice [（10.0±3.74） weeks，P<0.05]；The duration of serum HBsAg secretion in BALB/c mice injected with low dose（2.5μg） of pAAV/HBV1.2 plasmid was（3.67±1.03） weeks，significantly longer than that in high dose （50μg） group[（2.33±0.52） weeks，P<0.05]；On the tenth day after hydrodynamic injection，HBcAg special T lymphocytes appeared in the spleen of BALB/c mice. Conclusion HBV-infection mouse model is successfully established by hydrodynamic injection of pAAV/HBV1.2 plasmid and we find that mouse genetic background， immune response and viral doses will impact the establishment of this kind of HBV-infection mouse model.

Key words: Hepatitis B, pAAV/HBV1.2 plasmid, Hydrodynamic injection, Mice