Abstract: Objective To explore the changes of intestinal microecology in mice with xenograft hepatoma intervened by sodium norcantharidate.Methods Twelve Kunming mice were randomly divided into sodium nor-cantharidate-intervend,saline-intervend and control groups,and a mouse model of H22 hepatoma xenograft was es-tablished in sodium norcantharidate and saline groups. The mice were normally fed for 2 weeks after the success-ful establishment of the model. Then,the fecal samples were collected and Illumina miseq 2×300 bp high -throughput sequencing and bioinformatics analysis were performed.Results Every samples in each group con-tained abundant species,and species abundance and evenness tended to be consistent；there were significant differ-ences as respect to bacterial communities among sodium norcantharidate,saline and control group；at phylum,the most significantly different bacterial community was Proteobacteria and in sodium norcantharidate-intervend group,the proportion of Proteobacteria was 9.1%,significantly higher than those in saline group or the control group（2.8% and 2.3% ,respectively，P <0.05）;in genus，the different bacterial community included Bacteroides，etc；in saline-intervened group，the proportion of Bacteroides was 26.8%,significantly higher than those in sodium norcan-tharidate group or the control group （13.2% and 6.8%,respectively,P<0.05）. Conclusion There is a obvious change in intestinal microecology in mice with hepatoma xenograft receiving sodium norcantharidate intervention,which might be related to the anti-tumor effect of this agent.

Key words: Hepatoma, Sodium norcantharidate, Intestinal microecology, Mice