Abstract: Objective The aim of this study was to investigate the application of indocyanine green-mediated near-infrared light detection in patients undergoing cholecystectomy.Methods The clinical data of 45 patients with gallbladder carcinoma treated in our hospital between January 2016 and January 2018 were prospectively collected and analyzed. Intravenous injection of indocyanine green was given before surgery to guide finding the tumor fluorescence imaging during the operation. The anatomical features of the tumors in the isolated specimens were observed too.Results Forty-five tumors were found in 45 patients before operation,out of them,37 could show fluorescence in the surface of the tumors,and the depths and diameters of tumors with fluorescent manifestation were （0.4±0.2） cm and （6.3 ±2.1） cm,respectively,significantly different as compared to those in 8 tumors without fluorescent feature [（1.5±0.4） cm and （4.5±0.3） cm,respectively,P<0.05];in 45 patients,the in vitro tumor tissue specimens were cut open by the middle,and the sections showed strong fluorescence,among them,23 highly differentiated gallbladder carcinomas were with parenchymal imaging,4 out of 14 moderately differentiated carcinomas were with parenchymal imaging,and the other 8 poorly differentiated gallbladder carcinomas were without any imaging consistent with tumor surrounding tissue;at 1-year follow-up,no deaths were found,and 7 （15.6%） patients had tumor recurrence. Conclusion Indocyanine green-mediated near-infrared light imaging could show the location of the tumors,helping find the lesions that are commonly neglected by the routine examination.

Key words: Gallbladder carcinoma, Cholecystectomy, Indocyanine green, Near-infrared light fluorescent imaging, Therapy