Abstract: Objective To investigate the changes of peripheral blood 25-（OH）2-D3 levels and peripheral T lymphocyte subsets in patients with primary biliary cirrhosis（PBC） receiving vitamin D supplementation and ursodeoxycholic acid（UDCA） treatment. Methods 70 patients with PBC were recruited in our hospital between April 2013 and April 2016,and they were randomly divided into the observation group and the control group with 35 cases in each group by the random number generated by computer. The patients in control group was treated with UDCA,and those in the observation group was additionally treated with calcitriol soft capsules （vitamin D） at the base of UDCA. The response rate to UDCA were statistically analyzed. Results At the end of one year treatment,serum levels of 25-（OH）2-D3 and blood CD8⁺ cells in the observation group were （24.5±4.2） ng/ml and （29.0±5.2） %,respectively,significantly higher than （20.2±3.6） ng/ml and （26.3±4.8） %,while the percentage of CD4⁺ cells and the ratio of CD4⁺/CD8⁺ cells were （31.8±5.9） % and （1.1±0.2）,significantly lower than [（36.2±6.4） % and（1.4±0.3） in the control,all P<0.05];the response rate to UDCA by Barcelona criteria in the observation group was 85.7%,significantly higher than 62.9% in the control group （P<0.05）;serum diamine oxidase（DAO）,D-lactic acid,aspartate aminotransferase （AST） and alkaline phosphatase （ALP） levels in the observation group were （3.5±0.6） U/ml,（8.2±1.5） g/ml,（62.3±11.2） U/L and（317.5±57.3） U/L,significantly lower than [（3.9±0.7）U/ml,（9.4±1.7） g/ml,（76.9±13.9） U/L and（355.4±64.2） U/L in the control（P<0.05）. Conclusion Vitamin D supplementation can increase the response to UDCA,regulate the abnormal immune functions,protect intestinal barrier functions and promote liver function recovery in patients with PBC.

Key words: Primary biliary cirrhosis, Ursodeoxycholic acid, Vitamin D, T lymphocytes, Therapy