急性肝衰竭小鼠肝组织IL-25和IL-17受体mRNA水平变化

doi:10.3969/j.issn.1672-5069.2016.03.006

Abstract

Abstract: Objective To investigate the role of interleukin-25（IL-25） in acute liver failure in vivo. Methods An D-galactosamine and lipopolysaccharide-induced acute liver failure in mice was established. The relative levels of IL-25 and interleukin-17 receptor B（IL-17RB） messenger RNA in livertissues of mice were detected by real-time PCR and the IL-25 levels in homogenates of mice liver tissues was detected by enzyme-linked immunosorbent assay. Results The relative levels of IL-25 and IL-17RB mRNA in mice with acute liver failure [（18.05±2.46） and（1.89±0.4）] were significantly higher than in the controls （P<0.05）；the levels of IL-25 in liver tissue supernatants of mice with acute liver failure were （26.17±14.00） pg/ml，significantly higher than that in the controls[（12.50±4.68） pg/ml，P<0.05]. Conclusion IL-25 may play an important role in the pathogenesis of acute liver failure.

Key words: Acute liver failure, IL-25, IL-17 receptor, Mice

Liu Ziyun,Tian Deying,Wu Liang. Changes of IL-25 and IL-17RB mRNA in mice with D-galactosamine and lipopolysaccharide-induced acute liver failure[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2016, 19(3): 275-278.

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Changes of IL-25 and IL-17RB mRNA in mice with D-galactosamine and lipopolysaccharide-induced acute liver failure

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