TCRγδ+CD3+CD4-CD8-双阴性T细胞在3型鼠肝炎病毒诱导的小鼠慢性病毒性肝炎发病中的作用*

doi:10.3969/j.issn.1672-5069.2012.04.022

Abstract

Abstract: Objective The arm of this study was to investigate the changes of TCRγδ+CD3+CD4-CD8 （TCRγδ+DNT） cells in C3H/Hej mice with murine hepatitis virus type 3（MHV-3）- induced viral hepatitis. Methods MHV-3- induced viral hepatitis was established. At day 0,3,7,10,15 and 20 after MHV-3 infection,the TCRγδ+DNT cells in peripheral blood and spleen were counted by fluorescent antibodies staining and detected by flow cytometry for its proportion to CD3+T cells and the expression of CD25,CD28,CD30 and CD44 of the cells were identified. Results After MHV-3 infection,the proportion of TCRγδ+DNT to CD3+T cells in peripheral blood and spleen were markedly increased（P< 0.05） and it peaked at day 10（4.8± 1.5% and 4.5± 1.3% in peripheral blood and spleen,respectively）;The analysis of cell surface marker demonstrated that both of the phenotypes of TCRγδ+DNTs in peripheral blood and spleen were TCRγδ+CD3+CD4-CD8-CD25-CD28-CD30-CD44+. Conclusions The increase of TCRγδ+DNT in peripheral blood and spleen may play an important role in the pathological process of MHV-3-induced viral hepatitis.

Key words: Viral hepatitis, Murine hepatitis virus type-3, TCRγδ+CD3+CD4-CD8-double negative T cells, Mouse

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Changes of TCRγδ⁺CD3⁺CD4^-CD8^- double negative T cells in mice with MHV-3-induced viral hepatitis Lu Yulei,Wang Xiaojing,Yan Weiming,et al.

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Changes of TCRγδ+CD3+CD4-CD8- double negative T cells in mice with MHV-3-induced viral hepatitis Lu Yulei,Wang Xiaojing,Yan Weiming,et al.

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Changes of TCRγδ⁺CD3⁺CD4^-CD8^- double negative T cells in mice with MHV-3-induced viral hepatitis Lu Yulei,Wang Xiaojing,Yan Weiming,et al.