IL-22和SOCS3在3型鼠肝炎病毒诱导的小鼠慢性病毒性肝炎发病中的作用探讨*

doi:10.3969/j.issn.1672-5069.2014.04.017

Abstract

Abstract: Objective To investigate the changes of interleukin-22 （IL-22） and suppressor of cytokine signaling 3（SOCS3） mRNA in murine hepatitis virus 3（MHV-3） induced chronic viral hepatitis in C3H/Hej mice. Methods Chronic viral hepatitis in C3H/Hej mice was induced by intra-peritoneal injection of MHV-3 virus at dose of 100pfu. At day 0,5,10,15 and 20 post-infection,IL-22 and SOCS3 mRNA in the liver tissues were detected by real-time PCR. Results IL-22 and SOCS3 mRNA level in livers of model mice at day 0 were （0.26±0.15）% and（6.35±2.21）%,respectively. At day 10,15 and 20,IL-22 mRNA levels in the liver tissues were （6.28±2.79）%,（2.50±1.24）% and（2.73±0.85）%,respectively,and SOCS3 mRNA levels were（16.92±4.39）%,（14.06±4.09）% and （13.36±1.89）%,respectively, all of which were markedly increased than baseline（P<0.05）. The mRNA levels of IL-22 and SOCS3 both peaked at day 10 post MHV-3 infection. Conclusions IL-22 and SOCS3 may play an important role in the pathogenesis of MHV-3-induced chronic viral hepatitis in mice.

Key words: Murine hepatitis virus 3, Chronic viral hepatitis, Interleikin-22, Suppressor of cytokine signaling 3, Mouse

Li Yong, Lu Yulei, Wu Ting. Effect of IL-22 and SOCS3 in MHV-3 induced chronic viral hepatitis in mice[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2014, 17(4): 400-404.

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Effect of IL-22 and SOCS3 in MHV-3 induced chronic viral hepatitis in mice

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