四氯化碳联合高脂饮食对肝硬化家兔动脉粥样硬化的影响*

doi:10.3969/j.issn.1672-5069.2014.03.013

实用肝脏病杂志 ›› 2014, Vol. 17 ›› Issue (3): 271-275.doi: 10.3969/j.issn.1672-5069.2014.03.013

四氯化碳联合高脂饮食对肝硬化家兔动脉粥样硬化的影响^*

魏国华, 蔡晓波, 徐正婕, 范建高

200060 上海市普陀区人民医院消化内科(魏国华); 上海交通大学附属第一人民医院消化内科（蔡晓波）; 上海交通大学医学院附属新华医院消化内科（徐正婕,范建高）

收稿日期:2014-01-22 出版日期:2014-06-30 发布日期:2016-04-11
通讯作者: 范建高,E-mail：fanjiangao@gmail.com
作者简介:魏国华,女,33岁,医学硕士,主治医师。研究方向为脂肪肝与动脉硬化。E-mail：weiweidark@hotmail.com
基金资助:
国家重点基础研究发展计划973项目（2012CB517501）; 国家自然科学基金项目（81070322/81270491）; 上海市科学技术委员会科研基金项目（09140903500/10411956300）; 上海市卫生局新百人计划项目（XBR2011007）

Impact of carbon tetrachloride induced cirrhosis on high-fat diet related atherosclerosis in rabbits

Wei Guohua, Cai Xiaobo, Xu Zhenjie

Department of Gastroenterology,People’s Hospital,Putuo District,Shanghai 200060,China

Received:2014-01-22 Online:2014-06-30 Published:2016-04-11

摘要/Abstract

摘要： 目的在动物实验观察肝硬化对高脂饮食诱导的动脉硬化的影响。方法40只雄性新西兰大白兔被分为对照组（n=6）、高脂组（n=8）、四氯化碳（CCl₄）组（n=12）和高脂联合 CCl₄组（n=14）,采用CCl₄诱导肝硬化,观察高脂饮食诱导脂肪性肝损伤及动脉硬化,在10周末处死动物,比较血清转氨酶、血脂、空腹血糖以及肝脏和升主动脉形态学变化。结果高脂饮食组和复合模型组动物均成功复制出动脉硬化和脂肪性肝损伤模型;CCl4组动物血清ALT（87.42±57.92） IU/L和AST（137.33±114.54） IU/L均显著高于对照组[（21.33±17.76） U/L和（63.67±29.06） IU/L,P<0.05],伴明显的肝脏脂肪变、炎症及纤维化,而动脉内膜/中膜（I/M）比值及内-中膜厚度（IMT）无显著改变;与对照组比,高脂组和复合模型组动物血清转氨酶、血脂和血糖水平均显著升高（P<0.05）,肝脏脂肪变、炎症及纤维化程度明显,并且I/M比值及IMT显著升高;与高脂组比,复合模型组血清ALT[（169.63±88.08） IU/L 对（51.00±46.23） IU/L,P<0.05）]和肝纤维化半定量计分[（8.80±4.87）对（5.71±3.15）,P<0.05）]升高,而I/M比值则显著降低[（0.52±0.23）对（1.13±0.32）,P<0.01],但IMT仅略有下降[（226.01±51.38） μm 对（256.60±54.27）μm,P>0.05]。结论CCl₄与高脂饮食联合作用将加重家兔肝脏炎症损伤程度,但动脉硬化程度较单纯高脂饮食干预反而有所减轻。

关键词: 非酒精性脂肪性肝炎, 肝硬化, 动脉粥样硬化, 四氯化碳

Abstract: Objective To investigate the effect of cirrhosis on the development of atherosclerosis in vivo. Methods Normal male New Zealand white rabbits（n=40） were randomly divided into four groups,e.g. control （n=12）,high-fat （n=8）,carbon tetrachloride （CCl₄）（n=12） and combined treatment group （n=14）;Liver cirrhosis was induced by CCl4 injection and atherosclerosis by high fat diet（92% normal rabbit chow with 2% cholesterol and 6% lard） intake;Rabbits were sacrificed at the end of week 10 and serum aminotransferase,lipid,and fasting plasma glucose were examined and morphology changes in ascending aortas and livers were observed. ResultsAtherosclerosis was successfully induced by high-fat diet intake both in high-fat group and in combinational treatment group,while steatohepatitis-related cirrhosis was induced by CCl₄ administration and high fat diet intake in both groups;As compared with in control group,serum ALT and AST in CCl₄ group were increased significantly[（87.42±57.92）IU/L and （137.33±114.54）IU/L vs. （21.33±17.76） U/L and （63.67±29.06） IU/L,P<0.05],and significant liver steatosis,inflammation and fibrosis were also observed in CCl4-treated rabbits,but atherosclerosis were not found in ascending aortas;Serum aminotransferase,lipid and fasting plasma glucose in animals of high lipid diet were significantly higher than those in the controls（P<0.05）,in addition,significant liver steatosis,inflammation and fibrosis,and typical atherosclerosis plaques in ascending aortas were also observed in animals with high-fat diet intake;There were significant differences in serum alanine aminotransferase and intima-to-media （I/M） ratio of ascending aortas,but not in intima-media thickness（IMT）between the high fat group and the combinational treatment group. Conclusions A combination of high fat diet and CCl₄ aggravates liver inflammation but attenuates atherosclerosis induced by high fat diet in rabbits.

Key words: Nonalcoholic steatohepatitis, Cirrhosis, Atherosclerosis, Carbon tetrachloride

魏国华, 蔡晓波, 徐正婕, 范建高. 四氯化碳联合高脂饮食对肝硬化家兔动脉粥样硬化的影响^*[J]. 实用肝脏病杂志, 2014, 17(3): 271-275.

Wei Guohua, Cai Xiaobo, Xu Zhenjie. Impact of carbon tetrachloride induced cirrhosis on high-fat diet related atherosclerosis in rabbits[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2014, 17(3): 271-275.

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四氯化碳联合高脂饮食对肝硬化家兔动脉粥样硬化的影响^*

Impact of carbon tetrachloride induced cirrhosis on high-fat diet related atherosclerosis in rabbits

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