TCRγδ+CD3+CD4-CD8-双阴性T细胞在3型鼠肝炎病毒诱导的小鼠慢性病毒性肝炎发病中的作用*

doi:10.3969/j.issn.1672-5069.2012.04.022

摘要/Abstract

摘要： 目的本研究拟探讨在MHV-3诱导的慢性病毒性肝炎模型小鼠外周血和脾脏TCRγδ⁺DNT细胞在T淋巴细胞中比例变化以及其主要表面分子标记的变化。方法取60只C3H/Hej小鼠,腹腔注射10pfu MHV-3;应用流式细胞仪三色或四色荧光分析法分别检测小鼠外周血和脾脏TCRγδ⁺DNT细胞在T淋巴细胞中的比例及其CD25、CD28、CD30和CD44表达的变化。结果MHV-3诱导的慢性病毒性肝炎小鼠各时间点外周血和脾脏TCRγδ⁺DNT在T淋巴细胞中的比例较对照组显著升高（P<0.05）;在感染后10天达到最高峰：外周血和脾脏TCRγδ⁺DNT细胞占T淋巴细胞的比例分别为4.8±1.5%和4.5±1.3%;模型小鼠外周血和脾脏TCRγδ⁺DNT细胞的表型均为TCRγδ⁺CD3⁺CD4^-CD8^-CD25^-CD28^-CD30^-CD44⁺。结论感染MHV-3后C3H/Hej小鼠体内TCRγδ⁺DNT细胞的数量增加,提示TCRγδ⁺DNT细胞参与了MHV-3诱导的小鼠慢性病毒性肝炎的发生和发展过程。

关键词: 病毒性肝炎, 3型鼠肝炎病毒, TCRγ, δ, +DNT细胞, 小鼠

Abstract: Objective The arm of this study was to investigate the changes of TCRγδ+CD3+CD4-CD8 （TCRγδ+DNT） cells in C3H/Hej mice with murine hepatitis virus type 3（MHV-3）- induced viral hepatitis. Methods MHV-3- induced viral hepatitis was established. At day 0,3,7,10,15 and 20 after MHV-3 infection,the TCRγδ+DNT cells in peripheral blood and spleen were counted by fluorescent antibodies staining and detected by flow cytometry for its proportion to CD3+T cells and the expression of CD25,CD28,CD30 and CD44 of the cells were identified. Results After MHV-3 infection,the proportion of TCRγδ+DNT to CD3+T cells in peripheral blood and spleen were markedly increased（P< 0.05） and it peaked at day 10（4.8± 1.5% and 4.5± 1.3% in peripheral blood and spleen,respectively）;The analysis of cell surface marker demonstrated that both of the phenotypes of TCRγδ+DNTs in peripheral blood and spleen were TCRγδ+CD3+CD4-CD8-CD25-CD28-CD30-CD44+. Conclusions The increase of TCRγδ+DNT in peripheral blood and spleen may play an important role in the pathological process of MHV-3-induced viral hepatitis.

Key words: Viral hepatitis, Murine hepatitis virus type-3, TCRγδ+CD3+CD4-CD8-double negative T cells, Mouse

陆玉蕾, 王晓晶, 严伟明, 王洪武, 宁, 琴. TCRγδ⁺CD³⁺CD^4-CD^8-双阴性T细胞在3型鼠肝炎病毒诱导的小鼠慢性病毒性肝炎发病中的作用^*[J]. 实用肝脏病杂志, 2012, 15(4): 339-342.

参考文献

[1] D'Acquisto F,Crompton T. CD3 + CD4 - CD8 - （double negative）T cells:saviours or villains of the immune response?Biochem Pharmaco,2011,82（4）:333-340.
[2] Thomson CW,Lee BPL,Zhang L. Double-negative regulatory T cells-Non-conventional regulators. Immunol Res,2006,35（1-2）:163-177.
[3] Fischer K,Voelkl S,Przybylski GK,et al. Isolation and characterization of human antigen-specific TCR alpha beta（+）CD4（-）CD8（-）double negative regulatory T cells. Blood,2005,105（7）:2828-2835.
[4] 王晓晶,严伟明,张江国,等. CD4-CD8-T淋巴细胞在3型鼠肝炎病毒诱导的鼠慢性肝炎中的作用.中华肝脏病杂志,2009,3（17）：180-183.
[5] Rehermann B,Nascimbeni M. Immunology of hepatitis B virus and hepatitis C virus infection. Nat Rev Immunol,2005,5（3）:215-229.
[6] Ichiki Y,He XS,Shimoda S,et al. T cell immunity in hepatitis B and hepatitis C virus infection:implications for autoimmunity. Autoimmun Rev,2005,4（2）:82-95.
[7] Zou Y,Chen T,Han M,et al. Increased killing of liver NK cells by Fas/Fas ligand and NKG2D/NKG2D ligand contributes to hepatocyte necrosis in virus-induced liver failure. J Immunol,2010,184（1）:466-475.
[8] 邱洁,龙启强,冯艳红,等. 慢性乙型肝炎患者外周血NK细胞和T淋巴细胞计数变化及其临床意义.实用肝脏病杂志,2010,3（13）:178-179.
[9] 顾锡炳,杨小娟,王栋,等. 慢性乙型肝炎患者血清HBV DNA水平与外周血T淋巴细胞和NK细胞计数的关系. 实用肝脏病杂志,2010,6（13）:441-442.
[10] Zhang ZX,Yang L,Young KJ,et al. Identification of a previously unknown antigen-specific regulatory T cell and its mechanism of suppression. Nat Med,2000,6（7）:782-789.
[11] Seki S,Abo T,Masuda T,et al. Identification of activated T cell receptor gamma delta lymphocytes in the liver of tumor-bearing hosts. J Clin Investig,1990,86（2）:409-415.
[12] Gollob KJ,Antonelli LR,Faria DR,et al. Immunoregulatory mechanisms and CD4-CD8- （double negative）T cell subpopulations in human cutaneous leishmaniasis:a balancing act between protection and pathology. Int Immunopharmacol,2008,8（10）:1338-1343.
[13] Liu MF,Yang CY,Chao SC,et al. Distribution of double-negative（CD4 - CD8 - ,DN）T subsets in blood and synovial fluid from patients with rheumatoid arthritis. Clin Rheumatol,1999,18（3）:227-231.
[14] Beetz S,Wesch D,Marischen L,et al. Innate immune functions of human gamma delta T cells. Immunobiology,2008,2139（3-4）:173-182.
[15] Johnson P,Ruffell B. CD44 and its role in inflammation and inflammatory diseases. Inflamma Aller Drug Targ,2009,8（3）:208-220.
[16] Hanley WD,Napier SL,Burdick MM,et al. Variant isoforms of CD44 are P-and L-selectin ligands on colon carcinoma cells. FASEB J,2006,20（2）：337-339.
[17] Dustin M. A supercede for inflammation.Immunity,2004,20：361-362.
[18] 徐心,周娟,徐金锴,等. 门静脉高压症患者肝组织CD44血清透明质酸和肝功能性血流量的变化.西安交通大学学报（医学版）,2011,32（6）：739-741.

TCRγδ⁺CD³⁺CD^4-CD^8-双阴性T细胞在3型鼠肝炎病毒诱导的小鼠慢性病毒性肝炎发病中的作用^*

Changes of TCRγδ⁺CD3⁺CD4^-CD8^- double negative T cells in mice with MHV-3-induced viral hepatitis Lu Yulei,Wang Xiaojing,Yan Weiming,et al.

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