黄芩苷诱导人肝癌HepG-2细胞凋亡作用观察

doi:10.3969/j.issn.1672-5069.2012.04.023

实用肝脏病杂志 ›› 2012, Vol. 15 ›› Issue (4): 343-345.doi: 10.3969/j.issn.1672-5069.2012.04.023

黄芩苷诱导人肝癌HepG-2细胞凋亡作用观察

梁慧敏, 时小燕, 和振坤, 徐庆杰

收稿日期:2012-02-02 出版日期:2012-08-10 发布日期:2017-03-15
作者简介:第一作者：梁慧敏女,36岁,博士研究生,主治医师。主要从事肝癌发病机制研究。E-mail: luciasyl@163.com

Inhibition of apoptosis of HepG-2 cells by bacalin in vitro

Liang Huimin, Shi Xiaoyan, He Zhengkun et al.

Department of Infectious Diseases,Huaihe Hospital,Affiliated to Henan University,Kaifeng 475001,China

Received:2012-02-02 Online:2012-08-10 Published:2017-03-15

摘要/Abstract

摘要： 目的研究黄芩苷对人肝癌HepG-2细胞凋亡的影响,并探讨其作用机制。方法以不同浓度的黄芩苷与人肝癌HepG-2细胞共同培养,采用MTT法测定细胞增殖抑制率;采用Hoechst33258/PI染色法在荧光显微镜下观察凋亡细胞形态学变化;采用TUNEL法检测细胞凋亡率;采用Western blot法检测细胞凋亡相关蛋白Caspase-9、Caspase-3和Bcl-2表达的变化。结果在25~100μg/ml的药物浓度作用下,细胞增殖被抑制。药物浓度在50μg/ml下作用48h,细胞抑制率达50.63%,药物浓度在75μg/ml下作用48h细胞,抑制率达77.62%。抑制率呈浓度和时间依赖性;药物浓度在50μg/ml时作用48h,可见HepG-2细胞皱缩、细胞核碎裂成碎片,呈现典型的凋亡改变;随着黄芩苷作用浓度的增加,细胞凋亡率增高（P< 0.05）;随药物浓度的增加,Caspase-9和Caspase-3蛋白表达量呈增加趋势,而Bcl-2表达减少。结论黄芩苷能通过诱导细胞凋亡进而抑制人肝癌细胞增殖,其诱导凋亡机制可能与线粒体通路有关。

关键词: 肝癌, HepG2细胞, 黄芩苷, 细胞凋亡

Abstract: Objective To investigate the inhibition of apoptosis of HepG-2 cells by bacalin in vitro. MethodsHuman hepatocellular carcinoma cells,HepG-2 were cultured with different concentrations of baicalin and the inhibitory rate of cell proliferation was calculated with MTT assay;Morphology changes were detected by Hoechst33258/PI double fluorescence coloration;The apoptosis rate was detected by TUNEL;the Caspase-9,Caspase-3 and Bcl-2 protein expression were detected by Western blot. Results The cell proliferation was inhibited by baicalin at concentration of 25 to 100μg/ml;the inhibitory rate in 48h was 50.63% at 50μg/ml and 77.62% at 75μg/ml,and the inhibitory effect was in the concentration and time-dependent manner;there was a typical apoptosis at 50μg/ml in 48h,showing cell shrinkage and fragmentation of cell nucleus;the caspase-9 and caspase-3 expression increased and Bcl-2 decreased as the concentration of baicalin increased. Conclusion Baicalin can inhibit proliferation of HepG2 cells by inducing apoptosis and the mechanisms might be related to the mitochondrial pathway.

Key words: Hepatoma, HepG2 cells, Baicalin, Apoptosis

梁慧敏, 时小燕, 和振坤, 徐庆杰. 黄芩苷诱导人肝癌HepG-2细胞凋亡作用观察[J]. 实用肝脏病杂志, 2012, 15(4): 343-345.

Liang Huimin, Shi Xiaoyan, He Zhengkun et al.. Inhibition of apoptosis of HepG-2 cells by bacalin in vitro[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2012, 15(4): 343-345.

参考文献

[1] 李荣,李俊. 黄酮类化合物药理活性及其构效关系研究进展. 安徽医药,2005,9（7）:481.
[2] 汤立建,赵良才,李庆林,等. 黄芩黄酮类成分抗肿瘤作用及机制研究进展. 中国中药杂志,2007,32（1）: 21-26.
[3] Wang CZ,Li XL,Wang QF,et al. Selective fraction of Scutellaria baicalensis and its chemopreventive effects on MCF-7 human breast cancer cells. Phytomedicine,2010,17 （1）:63-68.
[4] Bonham M,Posakony J,Coleman I,et al.Characterization of chemical constituents in Scutellaria baicalensis with antiandrogenic and growth-inhibitory activities toward prostate carcinoma. Clin Cancer Res,2005,11（10）:3905-3914.
[5] 任学群,李宜雄. 黄芩素抗肿瘤机制研究进展. 河南大学学报:医学版,2010,29（3）:175-179.
[6] Wang W,Guo QL,You QD,et al. The anticancer activities of Wogonin in murine sarcoma S180 both in vitro and in vivo. Biol Pharm Bull,2006,29（6）:1132-1137.
[7] 王晓天,张建宾,雷英杰. 喹诺酮类抗肿瘤药物的研究进展. 中国药学杂志,2004,39（12）：890-894.
[8] 宋琳莉,孟庆刚. 黄芩的药理作用研究进展. 中华中医药学刊,2008,26（8）：1676-1678.
[9] 杨明,钟锐生,陈桂红. 黄芩苷对人鼻咽癌CNE-2Z细胞生长的抑制作用. 广东药学院学报,2011,27（4）:400-403.
[10] Kirkland D,Reeve L,Gatehouse D,et al. A core in vitro genotoxicity battery comprising the Ames test plus the in vitro micronucleus test is sufficient to detect rodent carcinogens and in vivo genotoxins. Mutat Res,2011,721（1）:27-73.
[11] Mantena SK. Ballga MS,Katiyar SK. Grape seed proanthocyanidins induce apoptosis and inhibit metastasis of highly metastatic breast carcinoma cells. Carcinogenesis,2006,27（8）:1682-1691.
[12] 刘萍,王菊英,许复郁,等. 黄芩苷对局灶性脑缺血再灌注损伤大鼠海马神经细胞凋亡的影响.中国药理学与毒理学杂志,2005,19（6）:412.
[13] 刘萍,李倩,刘兆平,等. 黄芩苷对脑缺血再灌注损伤大鼠神经细胞凋亡及相关基因表达的影响.中国新药与临床杂志,2007,26（2）:109.

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黄芩苷诱导人肝癌HepG-2细胞凋亡作用观察

Inhibition of apoptosis of HepG-2 cells by bacalin in vitro

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