乙型肝炎病毒相关性肝癌组织维生素D受体表达情况分析

doi:10.3969/j.issn.1672-5069.2018.02.023

Abstract

Abstract: Objective To explore the expression of vitamin D receptor（VDR） in cancerous tissues of patients with hepatitis B virus （HBV）-related hepatocellular carcinoma （HCC）. Methods The relative levels of VDR mRNA in liver tissues was detected by real-time PCR. VDR expression were determined by immunohistochemical method, and the relative expression of VDR protein was detected by Western blot. Results Immunohistochemistry showed that cancerous tissues, para-cancerous tissues, normal liver tissues expressed VDR equally. VDR was mainly expressed in the cytoplasm, and there was almost no expression in the nucleus. The RT-PCR detection showed that VDR mRNA in cancerous tissues was（2.77±0.30）, significantly higher than that in the para- cancerous tissues（1.62±0.21） or normal liver tissues[（1.57±0.19）, P<0.01];Western blot showed that the expression of VDR protein in cancerous tissues was（1.15±0.57）, significantly higher than that in the para- cancerous tissues（1.02±0.25） or in normal liver tissues[（0.37±0.11）, P<0.01]. Conclusion VDR is closely related to the development of hepatocellular carcinoma

Key words: Hepatoma, Vitamin D, Vitamin D receptor

Li Yuling, Guo Wenzheng, Zhang Weimin, et al. Expression of vitamin D receptor in cancerous tissues of patients with hepatitis B virus-related hepatocellular carcinoma[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2018, 21(2): 249-252.

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Expression of vitamin D receptor in cancerous tissues of patients with hepatitis B virus-related hepatocellular carcinoma

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