Abstract: Objective To study the protective effects of Huazhirougan granule on liver tissue injuries of mice with alcohol and lipopolysaccharide-induced alcoholic hepatitis. Methods Eighty mice were randomly divided into control，model，and low，middle and high dose of Huazhirougan granule，and Hugan tablet group. Except in the control group，the mice in other five groups had 56% alcohol by intragastric administration once daily，and intraperitoneal administration of lipopolysaccharide twice a week，and then intragastricly administered corresponding drugs in the afternoon for 10 weeks. The serum levels of ALT，AST，CK，γ-GT，ALP，TBIL，TG，TC，HDL-C and LDL-C were measured. The liver tissues were fixed by formaldehyde，routine paraffin section，and hematoxylin-eosin staining. Results The liver mass index，serum ALT and AST levels in the model group increased significantly [（5.77±0.67）%，（82.22±6.20） U/L and （93.43±17.30） U/L] vs. [（4.44±0.42） %，（35.83±3.84） U/L and （66.43±5.14） U/L，P<0.05] as compared with in control group; the serum levels of transaminase in the low，middle， and high dose of Huazhirougan granule group，and Hugan tablet group decreased significantly as compared to those in model group（P<0.001）;The serum CK levels in the low，middle，and high dose of Huazhirougan granule group，and Hugan tablet group were（118.93±10.15） U/L，（102.33±8.07） U/L，（119.45±19.26） U/L，（104.00±8.15） U/L，significantly decreased as compared with in model group [（227.50±50.10） U/L，P<0.001]；The serum bilirubin levels also decreased significantly as compared with in model group（P<0.01）. Conclusion Huazhirougan granule can protect the alcoholic liver injuries in mice with alcohol and lipopolysaccharide- induced alcoholic hepatitis.

Key words: Alcoholic hepatitis, Huazhi Rougan granule, Lipopolysaccharide, Mice