二乙基亚硝胺诱导肝癌小鼠肝组织胶原纤维的变化*

doi:10.3969/j.issn.1672-5069.2015.05.018

Abstract

Abstract: Objective To observe the change of collagen in the development of liver cancer in mice with N-nitrosodiethylamine（DEN）-induced liver cancer. Methods Forty BALB/c mice were fed with DEN solution at concentration of 30 mg/ml for routine beverage. Three mice were sacrificed at w 0，w 4，w 8，w 12，w 16，w 20 and week 24 after DEN solution feeding，and the liver tissues were collected each time and embedded in paraffin for pathological examination. The collagen fibers were stained by sirius red staining. The integrated optical density（IOD） of samples were obtained by Image-Pro Plus 6.0. Results The liver cancer was successfully induced at week 20 after DEN intervention as confirmed by pathological check-up；The IOD levels of collagen in liver tissues at week 4 was（97.610±18.640），significantly lower than at week 8[（377.054±63.668），P＜0.05]；the IOD at week 8 was （377.054±63.668），significantly lower than at week 12[（625.875±110.846），P＜0.05]；the IOD at the end of week 16 was（847.289±53.473），significantly lower than at week 20 [（1671.301±292.593），P＜0.01]；the IOD at week 20 was（1671.301±292.593），significantly lower than at week 24[（3968.450±138.949），P＜0.01]. Conclusions During the development of liver cancer，the amount of collagen fiber in liver tissues increases gradually， which might be involved in the carcinogenesis.

Key words: Liver neoplasm, N-nitrosodiethylamine, Collagen, Mice

Hu Jingfeng，Li Sanqiang，Wu Qinchuan，et al.. Changes of collagen in liver tissues of mice with N-nitrosodiethylamine-induced liver cancer[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2015, 18(5): 521-524.

References

[1] 刘勇，赛岩，刘冬梅，等. 小鼠原发性肝癌短期诱导模型的建立和评价. 军事医学，2011，35（3）:175-179．
[2] Parkin DM， Bray F，Ferlay J，et al. Gloabl canaer statistics，2002. CA Cancer J Clin，2005，55:74-108．
[3] 汪猛，秦凯健，杨梦琪，等. 原发性肝癌的中医药诊治研究进展.中国药导报，2015，21（3）:264-266.
[4] 刘红，文彬，陈丹，等. 人大肠癌癌旁不同部位组织细胞外基质分子和细胞极性分子表达差异比较.世界华人消化杂志，2015，23（1）:163-169．
[5] Brinckerhoff CE，Rutter JL，Benbow U. Interstitial collagenases as markers of tumor progression. Clin Cancer Res，2000，6:4823-4830．
[6] 李健，李彧，牛建昭，等. 胶原纤维与器官纤维化. 解剖学报，2003，34（6）:424-426.
[7] 黄峰，韦爱凌. 三种不同剂量二乙基亚硝胺诱癌效果比较. 陕西医学杂志，2013，42（3）:263-265．
[8] Shirakami Y，Gottesman ME，Blaner WS. Diethylnitrosamine induced hepatocarcinogenesis is suppressed in lecithin:retinol acyltransferase deficient mice primarily through retinoid actions immediately after carcinogen administration. Carcinogenesis，2012，33:268-274．
[9] Ogawa K. Molecular pathology of early stage chemically induced hepatocarcinogenesis. Pathol Int，2009，59:605-622．
[10] Kushida M，Kamendulis LM，Peat TJ，et al. Dose-related induction of hepatic preneoplastic lesions by diethylnitrosamine in C57BL/6 mice. Toxicol Pathol，2011，39:776-786．
[11] 朱艳志，孔宪炳，颜朗. 间断给药DEN诱导大鼠肝癌模型及病理研究. 重庆医科大学学报，2010，35（12）:1843-1846．
[12] Okubo HM，Tanaka N. Detection of serum and intrahepatic hepatocyte growth factor during DEN-induced carcinogenesis in the rat. Hepatol Res，2002，24（4）:385-394．
[13] Fukumasu H，Avanzo JL，Heider R，et a1. Prolective effects of guarana（Panllinia cupana Mart.var.Sorbilis） against DEN-induced DNA damage on mouse liver. Food Chem Toxicol，2006，44（6）:862-867．
[14] 姜幼纯，董奇男，肖邦良，等. 非坏死剂量二乙基亚硝胺诱发大鼠肝癌模型的研究. 华西医科大学学报，2001，32（4）:555-558．
[15] Richardson DB，Maclehose RF，Langholz B，et al. Hierarchical latency models for dose-time-response associations. Am J Epidemiol，2011，173:695-702．
[16] Sonuni NE，Noel A. Membrane type-matrix metalloproteinases and tumor progression. Biochimie，2005，87（3-4）:329-342．
[17] 徐列明.肝纤维化的研究进展.中华肝脏病杂志，2010，18（8）:563-565．
[18] Laitakari J，Stenback F. Collagen matrix in development and progression of experimentally induced respiratory neoplasmas in the henmer. Toxicol Pathol，2001，29（5）:514．
[19] Ushiki T. Collagen fibers，reticular fibers and elastic fibers. A comprehensive understanding from a morphological viewpoint. Arch Histol Cytol，2002，65（2）:109．
[20] Souza LF，Souza VF，Silva LD，et a1. Expression of basement membrane laminin in oral squamous cell carcinomas. Braz J Otorhinolaryngol，2007，73（6）:768．

Changes of collagen in liver tissues of mice with N-nitrosodiethylamine-induced liver cancer

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