靶向乙型肝炎病毒X区的siRNAs对HepG2.2.15细胞HBV复制的抑制作用*

doi:10.3969/j.issn.1672-5069.2010.04.004

Abstract

Abstract: Objective To observe the inhibition of hepatitis B viral replication in HepG2215 cells by siRNAs targeting hepatitis B virus X gene. Methods Four siRNAs against the X region of hepatitis B virus were chemically synthesized，and then were subjected to HepG2.2.15 cells by liposome-mediated transfection. The HBsAg and HbeAg in the supernatant were assayed by ELISA and HBV DNA by RT-PCR. Results At 30nmoL/L，the four siRNA had no obvious suppression in HBeAg and HbsAg expression（P>0.05），while at the concentration of 60nmoL/L and 90nmoL/L，the inhibition of siRNA-1 and siRNA-4 were statistically significant with the inhibition rates of 41% and 43%；the HBV DNA replication was obviously reduced. Conclusion The chemically synthesized siRNAs can effectively inhibit hepatitis B viral replication in vitro.

Key words: Hepatitis B virus, HepG2215 cells, RNA interfering, Transfection

ZONG Shufang，CHEN Yong，WANG Lijuan.et al.. Inhibition of hepatitis B viral replication in HepG2215 cells by siRNAs targeting hepatitis B virus X gene[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2010, 13(4): 253-255.

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Inhibition of hepatitis B viral replication in HepG2215 cells by siRNAs targeting hepatitis B virus X gene

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