原儿茶酸对刀豆蛋白A所致的免疫性肝损伤小鼠肝组织MDA、NO、SOD和GSH-PX表达的影响

doi:10.3969/j.issn.1672-5069.2021.03.005

Abstract

Abstract: Objective The aim of this study was to investigate the effects of protocatechuic acid (PA) on hepatic malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) activities in mice with concanavalin A (ConA)-induced immune liver injury.Methods 60 Kunming mice were randomly divided into control, model, low-dose, middle-dose and high-dose (2.5 mg.kg^-1.d^-1、5 mg.kg^-1.d^-1、10 mg.kg^-1.d^-1) of PA-intervened and 0.2 g.kg^-1.d^-1 of bifendate-intervened group, with 10 in each. The mice in control group and in model group were intragastricly given normal saline for 10 days, and in other groups were intragastricly given PA or bifendate for 10 days. One hour after the last administration, the ConA were injected through tail vein once to induce immune liver injury models. Serum interleukin-4 (IL-4), IL-6, tumor necrosis factor-α (TNF-α) and the MDA, NO, SOD and GSH-PX activities in hepatic homogenate were assayed.Results The liver indexes, spleen indexed, serum ALT and AST, serum IL-4, IL-6 and TNF-α in any dose of PA-intervened groups decreased significantly compared to those in the model (P<0.05); the hepatic MDA and NO activities in model group were (5.2±0.5)nmol/mg and (8.0±0.9)μmol/L, while they both decreased to [(4.7±0.4)nmol/mg, (4.3±0.3)nmol/mg and (3.9±0.3)nmol/mg or (6.8±0.8)μmol/L,(6.2±0.7)μmol/L and(5.8±0.7)μmol/L, respectively, P<0.05] in low-dose, middle-dose and large-dose of PA-intervened groups; the hepatic SOD and GSH-PX activities in model were (59.4±3.6)U/mg and(85.2±9.6)U/mg, while they both increased to [(74.2±4.4)U/mg,(85.2±5.3)U/mg and (99.2±5.9)U/mg or(107.3±13.4)U/mg,(115.2±12.8)U/mg and (139.3±12.9)U/mg, respectively, P<0.05] in low-dose, middle-dose and large-dose of PA-intervened groups.Conclusion PA could alleviate ConA-induced liver injury in mice, which might be related to the increased activity of endogenous antioxidant enzymes in liver tissues.

Key words: Immune liver injury, Protocatechuic acid, Concanavalin A, Oxygen free radical, Mice

Wang Lihui, Zhang Jiaxiang , Liu Shuangping, et al. Alleviation of ConA-induced liver injury by protocatechuic acid in mice[J]. Journal of Practical Hepatology, 2021, 24(3): 323-326.

References

[1] 池肇春. 非酒精性脂肪性肝炎肝硬化发病机制研究进展与展望. 实用肝脏病杂志, 2018, 21(2):166-169.
[2]Khan HA, Ahmad MZ, Khan JA, et al. Crosstalk of liver immune cells and cell death mechanisms in different murine models of liver injury and its clinical relevance. Hepatobiliary Pancreat Dis Int, 2017, 16(3):245-256.
[3] Sánchez M, Castiella A, Zapata E, et al. Autoimmunehepatitis (immune-mediated liver injury) induced by rosuvastatin. Gastroenterol Hepatol, 2018, 41(5):311-313.
[4] Gutierrez-Zetina SM, Gonzalez-Manzano S, Perez-Alonso JJ, et al. Preparation and characterization of protocatechuic acid sulfates. Molecules, 2019, 24(2):307.
[5] Krzysztoforska K, Mirowska-Guzel D, Widy-Tyszkiewicz E. Pharmacological effects of protocatechuic acid and its therapeutic potential in neurodegenerative diseases: Review on the basis of in vitro and in vivo studies in rodents and humans. Nutr Neurosci, 2019, 22(2):72-82.
[6] Guan S, Bao YM, Jiang B, et al. Protective effect of protocatechuic acid from Alpinia oxyphylla on hydrogen peroxide-induced oxidative PC12 cell death. Eur J Pharmacol, 2006, 538(1-3):73-79.
[7] Guan S, Jiang B, Bao YM, et al. Protocatechuic acid suppresses MPP⁺-induced mitochondrial dysfunction and apoptotic cell death in PC12 cells. Food Chem Toxicol, 2006, 44(10):1659-1666.
[8] Li CJ, Yang ZH, Shi XL, et al. Effects of aspirin and enoxaparin in a rat model of liver fibrosis. World J Gastroenterol, 2017, 23(35):6412-6419.
[9] Fu Q, Yan S, Wang L,et al. Hepatic NK cell-mediated hypersensitivity to ConA-induced liver injury in mouse liver expressing hepatitis C virus polyprotein. Oncotarget, 2016, 8(32):52178-52192.
[10] 温韬, 刘焱, 任锋, 等. 刀豆蛋白A诱导的急性肝损伤小鼠血浆细胞外组蛋白的变化及意义. 实用肝脏病杂志, 2015, 18(3):278-281.
[11] Shi B, Feng ZQ, Li WB, et al. Low G preconditioning reduces liver injury induced by high +Gz exposure in rats. World J Gastroenterol, 2015, 21(21):6543-6549.
[12] Fayed MR, El-Naga RN, Akool ES, et al. The potential antifibrotic impact of apocynin and alpha-lipoic acid in concanavalin A-induced liver fibrosis in rats: Role of NADPH oxidases 1 and 4. Drug Discov Ther, 2018, 12(2):58-67.
[13] Suyavaran A, Ramamurthy C, Mareeswaran R, et al. TNF-α suppression by glutathione preconditioning attenuates hepatic ischemia reperfusion injury in young and aged rats. Inflamm Res, 2015, 64(1):71-81.
[14] Yu Q, Liu T, Li S, et al. The protective effects of levo-tetrahydropalmatine on ConA-induced liver injury are via TRAF6/JNK signaling. Mediators Inflamm, 2018, 2018:4032484.
[15] Ochiai S, Jagot F, Kyle RL, et al. Thymic stromal lymphopoietin drives the development of IL-13⁺ Th2 cells. Proc Natl Acad Sci U S A, 2018, 115(5):1033-1038.
[16] Wu GJ, Lin YW, Chuang CY, et al. Liver nitrosation and inflammation in septic rats were suppressed by propofol viadownregulating TLR4/NF-κB-mediated iNOS and IL-6 gene expressions. Life Sci, 2018, 195:25-32.
[17] 王新伟, 吕柯, 孙晓, 等. 选择性β3肾上腺素能受体激动剂对非酒精性脂肪性肝病大鼠血清氧化应激指标和肝组织纤维化的影响.实用肝脏病杂志, 2018, 21(3):384-387.
[18] 王建超, 银艳桃, 文彬,等. 天然牛磺酸抗肝纤维化作用机制研究. 中华中医药学刊, 2020, 38(1):144-147.
[19] 王玮, 赵彩彦, 王亚东, 等. 脂联素对转化生长因子β1致肝星状细胞低表达内皮型一氧化氮合酶的阻断作用. 中华肝脏病杂志, 2011,20(12):917-922.
[20] Fu R, Zhou JJ, Wang RW, et al. Protocatechuic acid-mediated miR-219a-5p activation inhibits the p66shc oxidant pathway to alleviate alcoholic liver injury. Oxid Med Cell Longev,2019, 2019:3527809.

Alleviation of ConA-induced liver injury by protocatechuic acid in mice

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