慢加急性肝衰竭小鼠肝组织组蛋白去乙酰化酶mRNA水平变化

doi:10.3969/j.issn.1672-5069.2021.03.006

Abstract

Abstract: Objective The aim of this study was to study the changes of histone deacetylase mRNA levels after histone acetylation (HDAC) inhibitor intervention in liver tissue of mice with acute-on-chronic liver failure (ACLF).Methods 28 mice were randomly divided into seven group, with four in each, and the liver failure was induced in mice by lipopolysaccharide (LPS) and D-Gal injection. The mice in group A (control), B (model), C, D, E, F and G were intervened by normal saline, normal saline, trichostatin A(TSA), large dose of sodium butyrate, low dose of sodium butyrate, large dose of pyrrolidine dithiocarbarmate (PDTC) and low dose of PDTC, respectively. The hepatic HDAC mRNA was assayed by Q-PCR.Results The hepatic HDAC1mRNA, HDAC2mRNA, HDAC3mRNA and HDAC8mRNA in group B were (1.7±0.2), (1.7±0.3), (1.9±0.6) and (2.6±0.7), while all of them in group C, group D, group E, group F and group G decreased greatly (P<0.05); the hepatic HDAC4mRNA, HDAC5mRNA, HDAC6mRNA, HDAC7mRNA, HDAC9mRNA and HDAC10mRNA in group B were (0.6±0.2),(6.3±0.9),(3.4±0.8),(2.8±1.0),(6.5±1.1) and (0.4±0.1), while all of them in group C, group D, group E, group F and group G decreased significantly as compared to those in group B(P<0.05).Conclusion The hepatic HDAC mRNA levels is related to the acetylation of proteins, which might reinforce the theoretical basis for the application of histone acetylation regulation in Dealing withliver failure in mice.

Key words: Acute-on-chronic liver failure, Histone deacetylase, Trichostatin A, Sodium butyrate, Pyrrolidine dithiocarbarmate, Mice

Lin Liekun, Lu Chunsheng, Zhou Yingsheng, et al. Changes of histone deacetylase mRNA levels after histone acetylation inhibitor management in liver tissue of mice with acute-on-chronic liver failure[J]. Journal of Practical Hepatology, 2021, 24(3): 327-330.

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Changes of histone deacetylase mRNA levels after histone acetylation inhibitor management in liver tissue of mice with acute-on-chronic liver failure

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