肝细胞癌癌组织Hic-5和COL1A1表达及其对术后生存的预测价值*

doi:10.3969/j.issn.1672-5069.2020.04.031

Abstract

Abstract: Objective The aim of this study was to analyze the expression of hydrogen peroxide inducible clone-5 (Hic-5) and collagen type I alpha gene (COL1A1) in cancerous tissues and their predictive value for postoperative survival time of patients with hepatocellular carcinoma (HCC). Methods 97 patients with HCC underwent hepatectomy in our hospital during the period between September 2013 and July 2016, and all patients were followed-up for 36 months. The RT-PCR and immunohistochemistry were performed to detect Hic-5 and COL1A1 mRNA and their proteins in cancerous and non-cancerous tissues. Results The positive rate of Hic-5 protein in cancerous tissues was significantly higher than that in adjacent liver tissues (53.6% vs. 16.5, P<0.05), and the positive rate of Hic-5 mRNA was significantly higher than that in adjacent tissues (66.0% vs. 25.8%, P<0.05); the positive rate of COL1A1 protein in cancerous tissues was significantly higher than that in adjacent tissues (71.1% vs. 25.8%, P<0.05), and the positive rate of COL1A1 mRNA was significantly higher than that in adjacent tissues (78.4% vs. 30.9%, P<0.05); the positive rate of Hic-5 mRNA in patients with TNM stage I/II was significantly lower than that in with stage III/IV (56.4% vs. 78.6%, P<0.05), and the positive rate of COL1A1 mRNA was significantly lower than that in with stage III/IV (70.9% vs. 88.1%, P<0.05); the positive rate of Hic-5 in patients with portal vein thrombosis was significantly higher than that in without (82.5% vs. 54.4%, P<0.05), and the positive rate of COL1A1 mRNA was significantly higher than that in without (90.9% vs. 70.2%, P<0.05); the medium survival in 87 followed-up patients was 8.2 to 36.0 months[36.0(14.6,36.0) m; the 3-year cumulative survival in patients with positive Hic-5 expression was significantly shorter than that in with negative [26.3(16.1,36.0) m vs. 36.0(30.6,36.0) m, P<0.05], and the 3-year cumulative event-free survival was also significantly shorter than that in with negative [21.4(11.7,36.0) m vs. 36.0(24.2,36.0]m, P<0.05). Conclusion The positive Hic-5 expression in cancerous tissues in patients with HCC hint poor prognosis, which might be related to the advanced tumor stage and portal vein thrombosis.

Key words: Hepatoma, Hepatectomy, Hydrogen peroxide inducible clone-5, Collagen type I alpha gene, Survival

Gu Shiyu, Qin Wenju, Gao Jianzhi, et al. Poor prognosis of patients withhepatocellular carcinoma after hepatectomy with high cancerous tissue Hic-5 and COL1A1 expression[J]. Journal of Practical Hepatology, 2020, 23(4): 576-580.

References

[1]Macaron C, Hanouneh I A, Zein N N. Incidence and risk factors of hepatocellular carcinoma in patients with primary biliary cirrhosis. Hepatology, 2015, 52(6):2240-2240.
[2]Yoon Y I, Kim K H, Kang S H, et al. Purelaparoscopic versus open right hepatectomy for hepatocellular carcinoma in patients with cirrhosis: A propensity score matched analysis. Ann Surg, 2017, 265(5):856-863.
[3]Ally A, Balasundaram M, Carlsen R, et al. Comprehensive andintegrative genomic characterization of hepatocellular carcinoma. Cell, 2017, 169(7):1327-1341.
[4]Gulvady A C, Dubois F, Deakin N O, et al. Hic-5expression is a major indicator of cancer cell morphology, migration and plasticity in three-dimensional matrices. Mol Bio Cell, 2018, 29(13):1704-1717.
[5]Li J, Ding Y, Li A. Identification of COL1A1 and COL1A2 as candidate prognostic factors in gastric cancer. World J Surg Oncol, 2016, 14(1):297-301.
[6]罗程, 陈琪, 方程, 等. Hic-5基因在胆管纤维化中的表达. 实用医学杂志, 2018, 34(20):51-55.
[7]Tao R, Fan X, Yu H, et al. MicroRNA-29b-3p prevents Schistosoma Japonicum‐induced liver fibrosis by targeting COL1A1 and COL3A1. J Cell Biochem, 2018, 8(1):102-110.
[8]Pirisi M, Leutner M, Pinato D J, et al. Reliability and reproducibility of the edmondson grading of hepatocellular carcinoma using paired core biopsy and surgical resection specimens. Arch Pathol Lab Med, 2010, 134(12):1818-1822.
[9]张杰, 段伯焕, 朱功兵. 肝细胞癌患者外科手术根治术后生存分析. 实用肝脏病杂志, 2017, 20(1):93-96.
[10]Sheta R, Wang Z Q, Bachvarova M, et al. Hic-5 regulates epithelial to mesenchymal transition in ovarian cancer cells in a TGFβ1-independent manner. Oncotarget, 2017, 8(47):82506-82530.
[11]Lei X F, Fu W, Kim-Kaneyama J R, et al. Hic-5 deficiency attenuates the activation of hepatic stellate cells and liver fibrosis through upregulation of Smad7 in mice. J Hepatol, 2016, 64(1):110-117.
[12]张金莲, 蔡兆根, 吴琼, 等. 肝细胞癌患者血清和肝组织microRNAlet-7水平及其与临床预后的关系. 实用肝脏病杂志, 2018, 21(3):119-122.
[13]Schleit J, Bailey S S, Tran T, et al. Molecularoutcome, prediction, and clinical consequences of splice variants in COL1A1, which encodes the proα1(I) chains of type I procollagen. Hum Mutat, 2015, 36(7):728-739.
[14]Zhang Z, Wang Y, Zhang J, et al. COL1A1 promotes metastasis in colorectal cancer by regulating the WNT/PCP pathway. Mol Med Rep, 2018, 17(4):1-6.
[15]Jolly L A, Novitskiy S V, Owens P, et al. Fibroblast-mediated collagen remodeling within the tumor microenvironment facilitates progression of thyroid cancers driven by BrafV600E and Pten loss. Cancer Res, 2016, 76(7):1804-1813.
[16]Nick H, Andrew M. Extracellular matrix-induced Hic-5 expression in glomerular mesangial cells leads to a prosclerotic phenotype independent of TGF-β. Faseb J, 2015, 29(12):4956-4967.
[17]Zhang S, Gong Y, Xiao J, et al. A COL1A1promoter-controlled expression of TGF-β soluble receptor inhibits hepatic fibrosis without triggering autoimmune responses. Dig Dis Sci, 2018, 63(10):2662-2672.
[18]Goreczny G J, Forsythe I J, Turner C E. Hic-5regulates fibrillar adhesion formation to control tumor extracellular matrix remodeling through interaction with tensinl. Oncogene, 2018, 37(13):1699-1713.
[19]Shen L, Yang M, Lin Q, et al. COL11A1 is overexpressed in recurrent non-small cell lung cancer and promotes cell proliferation, migration, invasion and drug resistance. Oncol Rep, 2016, 36(2):877-885.
[20]Omata M, Cheng AL, Kokudo N, et al. Asia–Pacific clinical practice guidelines on the management of hepatocellular carcinoma: a 2017 update. Hepatol Int, 2017, 11(4): 317–370.

Poor prognosis of patients withhepatocellular carcinoma after hepatectomy with high cancerous tissue Hic-5 and COL1A1 expression

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Zhang Jing, Zhou Xinmin. Administration of recombinant human thrombopoietin before operation in patients with liver cirrhosis and hepatocellular carcinoma and thrombocytopenia [J]. Journal of Practical Hepatology, 2020, 23(4): 552-555.
[2]	Meng Haisheng, Hu Dongmei, Wang Haitao, et al. Application of ultrasound single mode fusion imaging in the immediate evaluation of microwave ablation in treatment of patients with primary liver cancer [J]. Journal of Practical Hepatology, 2020, 23(4): 572-575.
[3]	Quan Yaning, Shang Xiaojie, Meng Xuan, et al. Application of microwave ablation in treatment of patients with small primary liver cancer under real-time contrast-enhanced ultrasound and fusion image navigation guidance [J]. Journal of Practical Hepatology, 2020, 23(4): 581-584.
[4]	Feng Tao, Hu Wei, Teng Buwei, et al. Value of ALBI and future liver remnant combinationin predicting the occurrence of post-hepatectomy liver failure in patients with hepatocellular carcinoma [J]. Journal of Practical Hepatology, 2020, 23(4): 585-588.
[5]	Wu Jie, Xie Lixiang, Xiu Jin. The value of US, CT and MRI in the evaluation of tumor activity in patients with primary liver cancer after TACE intervention [J]. Journal of Practical Hepatology, 2020, 23(3): 419-422.
[6]	Xu Xueqing, Li Bingchao,Feng Kaili. Clinical value of enhanced CT texture analysis technique in predicting short- term recurrence of liver tumor in patients with HCC after TACE [J]. Journal of Practical Hepatology, 2020, 23(3): 423-426.
[7]	Wang Liangliang,Yang Bo,Zhang Weiguo. Comparison of fluorescent laparoscopic hepatectomy and conventional laparoscopic hepatectomy in the treatment of patients with HCC [J]. Journal of Practical Hepatology, 2020, 23(3): 427-430.
[8]	Wang Wei, Du Xiaofei, Lu Yunjian. Predicting value for survival by preoperative NLR of patients with massive hepatocellular carcinoma treated by TACE combined with MWA [J]. Journal of Practical Hepatology, 2020, 23(2): 260-263.
[9]	Li Ying, Huang Haiqing, Xue Fushan, et al. Clinical observation of remifentanil combined with propofol target-controlled infusion anesthesia in patients with primary liver cancer underwent partial hepatectomy [J]. Journal of Practical Hepatology, 2020, 23(2): 272-275.
[10]	Tang Hao, Song Wenyuan, Zeng Kai. Survival of patients with severe traumatic hepatic rupture after damage control surgery [J]. Journal of Practical Hepatology, 2020, 23(2): 280-283.
[11]	Ning Baoshuo, Li Yiling. Effect of PNPLA3 on lipid metabolism of hepatocytes and hepatocellular carcinoma cells [J]. Journal of Practical Hepatology, 2020, 23(2): 300-304.
[12]	Chen Liang, Duan Haifeng, Yu Hongxing. Detection of tumor foci blood supply by SMI, CDFI and enhanced CT in patients with primary liver cancer after TACE [J]. Journal of Practical Hepatology, 2020, 23(1): 90-93.
[13]	Ma Ning, Dai Xiaowen , Wu Jianjiang. Influence of different types of liquid infusion on clinical efficacy and safety of patients withhepatocellular carcinoma undergoing partial hepatectomy [J]. Journal of Practical Hepatology, 2020, 23(1): 102-105.
[14]	Qian Guowu, Cui Guangying, Song Zhan, et al. Changes of blood cortsisol and peripheral blood T lymphocyte subsets in patients with complicated intrahepatic bile duct stones after laparoscopic regular hepatectomy or lobectomy [J]. Journal of Practical Hepatology, 2020, 23(1): 122-125.
[15]	Xu Ling, Ren Feng. Roles ofhepatic macrophages in several common liver diseases [J]. Journal of Practical Hepatology, 2020, 23(1): 145-148.