Abstract: Objective To analyze the correlation between serum and fecal HBV DNA and biochemical indexes and intestinal flora in patients with chronic hepatitis B（CHB）. Methods 73 patients with CHB were recruited in our hospital between August 2015 and March 2017,and liver function index,serum HBV markers and HBV DNA were detected. Besides,fecal HBV DNA and Bifidobacterium,Lactobacillus,Escherichia Coli, Enterococcus,Clostridium,Candida albicans,Bacteroides,Poulet,Rumen bacteria aureus in intestinal flora were assayed. The correlation between serum and fecal HBV DNA,and liver function index and intestinal flora DNA were compared. Results Fecal and serum HBV DNA levels in 48 patients with serum HBeAg positive were （5.9±1.6） copies/ml and （7.2±1.6） copies/ml,respectively,much higher than （5.0±1.9） copies/ml and （6.1±1.3） copies/ml （t=4.401,P=0.024;t=5.936,P=0.008） in 25 patients with serum HBeAg negative;there were no significant differences as respect to serum and fecal HBV DNA levels among 21,47 and 5 patients with mild, moderate and severe hepatitis B（P>0.05）;the fecal HBV DNA level was positively correlated to serum HBV DNA（r=0.61,P=0.002）;fecal HBV DNA was negatively correlated serum ALP and TBIL（r=-0.49,r=-0.54,P<0.05）,and serum HBV DNA load was negatively correlated to serum ALT and AST levels（r=-0.46,P=0.023;r=-0.52,P=0.008）;Fecal HBV DNA level was positively correlated to fecal enterococci（r=0.49,P=0.005） and serum HBV DNA was negatively correlated with lactobacillus（r=-0.53,P<0.001）. Conclusion The fecal HBV DNA is positive in patients with CHB,which warrants further investigation.

Key words: Hepatitis B, HBV DNA, Feces, Intestinal flora