Abstract: Objective To investigate the effect of 1aparoscopic hepatectomy on peripheral blood T lymphocyte subsets and serum cytokines in patients with primary liver cancer（PLC）. Methods 68 patients with PLC were recruited in our hospital between February 2014 and February 2015,and were randomly divided into two groups with 34 in each. 34 patients received laparoscopic hepatectomy and another 34 received open hepatectomy. The peripheral blood T lymphocyte subsets and serum cytokines,immunoglobulin levels were assayed and compared between the two groups. Results The percentages of peripheral blood CD3⁺,CD4⁺ and CD8⁺T cells in the two groups at presentation were not significantly different（P>0.05）,while the CD3⁺,CD4⁺ and CD8⁺T cells in patients receiving laparoscopic hepatectomy seven days after operation were（57.2±5.6）%,（46.9±7.2）% and （28.4±5.3） %,significantly different as compared to[（51.9±3.2）%,（41.2±6.4）% and（33.6±2.3）%] in open hepatectomy,respectively,（P<0.05）;serum vascular endothelial growth factor（VEGF）,fibroblast growth factors-a （aFGF） and bFGF levels were （18.4±5.1） ng/ml,（5.3±4.2） pg/ml and （6.5±4.3） pg/ml,much lower than [（31.9±5.7） ng/ml,（8.7±5.2） pg/ml and（8.4±6.9） pg/ml,respectivley,in open hepatectomy,P<0.05];the changes of blood liver function index and immumoglobulin levels in the two groups were not significantly different（P>0.05）. Conclusion The impact of laparoscopic hepatectomy on immune functions is milder than open operation, might be related to its mild injuries in the process.

Key words: Hepatoma, Laparoscopy hepetectomy, Peripheral blood T lymphocyte subsets, Vascular endothelial growth factor, Fibroblast growth factors