小分子干扰RNA沉默高迁移率族蛋白B1抑制肝星状细胞Ⅰ型和Ⅲ型胶原合成*

doi:10.3969/j.issn.1672-5069.2015.04.002

Abstract

Abstract: Objective To investigate the effects of synthetic small interfering RNA（siRNA） targeting high mobility group box chromosomal protein 1（HMGB1-siRNA） on collagen type Ⅰand Ⅲ in hepatic stellate cells （HSCs） in vitro. Methods Synthetic siRNA targeting HMGB1 was transfected into HSC-T6 cells by lipofectamine， and negative siRNA transfection and no transfection were used as negative control and blank control， respectively. After incubation with siRNA，total RNA and protein of HSC-T6 cells were extracted；the protein levels of typeⅠand Ⅲ collagen were determined by using Western blotting and immunofluorescence；Collagen type Ⅰand Ⅲ levels in the supernatants were determined by enzyme linked immunosorbent assay. Results The expression of collagen type Ⅰ and Ⅲ were significantly reduced after transfection of HMGB1-siRNA compared with in control or blank. The levels of collagen type I and type Ⅲ in the supernatants of transfected cells were decreased than in the negative control at 72 hours；the 1evels of type I collagen decreased by（46.36±3.82）% （F=33.14，P<0.01） and the 1evel of type Ⅲ collagen decreased by（41.92±3.58）%（F=27.56，P<0.01） at 72 hours when compared to the control. Conclusion Inhibition of HMGB1 by siRNA decreases the synthesis and the secretions of typeⅠ and Ⅲ collagen in HSCs and this might has a potential effect in prevention and treatment of hepatic fibrosis.

Key words: Hepatic stellate cells, Small interfering RNA, High mobility group box chromosomal protein 1, Collagen

Ge Wensong，Fang Jiangao， Li Guangming. Inhibition of small interfering RNA targeting high mobility group box chromosomal protein 1 on collagen type Ⅰand Ⅲ in hepatic stellate cells in vitro[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2015, 18(4): 340-343.

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Inhibition of small interfering RNA targeting high mobility group box chromosomal protein 1 on collagen type Ⅰand Ⅲ in hepatic stellate cells in vitro

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