针对STAT3的siRNA抑制HepG2细胞增殖和p44/42MAPK蛋白的表达*

doi:10.3969/j.issn.1672-5069.2010.06.001

Abstract

Abstract: Objective To study the effect of STAT3 signaling pathways on p44/42MAPK and cell growth in hepatocellular carcinoma. Methods The siRNA directed against STAT3 was transfected into HepG2 cells. Cell growth was measured by MTT assay and STAT3 and p44/42MAPK protein expression were measured by Western blot. Results A significant decrease in the number of viable cells（q=9.36，P<0.05）was found in the siRNA transfection group as compared to that in the control or the LipofectamineTM2000 transfected groups；SiRNA inhibited cell growth with the inhibition rates of 33.2% at 24h，39.6% at 48h，43.1% at 72h，and 33.9% at 96h；At 72h and 96h after the transfection，STAT3 protein expression was inhibited and P44/42MAPK protein expression was not influenced and p-p44/42MAPK protein expression was increased.Conclusion siRNA against STAT3 may affect cell growth in vitro.

Key words: HepG2 cell, RNA interference, Signal transducers and activators of transcription 3, p44/42MAPK, Signaling pathway

WANG Xinhong，XING Hui，CHEN Jing，et al.. Inhibition of cell growth and p44/42MAPK protein expression by siRNA against STAT3 in HepG2 cells in vitro[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2010, 13(6): 401-403.

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Inhibition of cell growth and p44/42MAPK protein expression by siRNA against STAT3 in HepG2 cells in vitro

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