Abstract: Objective To study the expression of Notch signaling pathway influenced by ethanol in C3A cells in vitro. Methods C3A cells were served as the control group,and the CYP2E1 cells were as experiment group. Equal concentration of ethanol and Notch signaling pathway inhibitor were added to the two groups of cells. RT-PCR and Western blotting were used to testify the expression of Notch1,NICD1 and HES1,respectively. MTT assay was used to evaluate the viability of cells. Results The cell viability in the two groups was decreased by ethanol,while the Notch signaling pathway inhibitor improved the viability of cells; The expression of NICD1 and HES1 was up-regulated in the experimental group,while when Notch signaling inhibitor was added into the medium,the expression of NICD1 and HES1 was significantly down-regulated; The Notch1 mRNA level was also up-regulated in the experimental group. Conclusion Ethanol can activate the expression of Notch signaling pathway in C3A models.

Key words: C3A cells, Ethanol, Notch signaling pathway