中药肝复康对肝星状细胞mTOR/P70S6K信号通路的干预作用

doi:10.3969/j.issn.1672-5069.2012.01.012

实用肝脏病杂志 ›› 2012, Vol. 15 ›› Issue (1): 24-25.doi: 10.3969/j.issn.1672-5069.2012.01.012

中药肝复康对肝星状细胞mTOR/P70S6K信号通路的干预作用

张坤, 姜妙娜, 张彩华, 贾玉杰

116044 辽宁省大连市大连医科大学病理生理学教研室

收稿日期:2011-07-25 出版日期:2012-02-10 发布日期:2016-05-11
通讯作者: 贾玉杰,E-mail:pathophy@163.com
作者简介:张坤女,32岁,讲师。主要从事消化系统疾病病理生理学研究。原工作单位为包头医学院。E-mail:kunkunlove0508@163.com

Effect of Chinese traditional medicine Ganfukang on the mTOR/P70S6K signal pathway in liver stellate cells

Zhang Kun, Jiang Miaona, Zhang Caihua, et al

Department of Pathophysiology,Dalian Medical University,Dalian 116044,Liaoning Province,China

Received:2011-07-25 Online:2012-02-10 Published:2016-05-11

摘要/Abstract

摘要： 目的观察中药肝复康含药血清对乙醛刺激的肝星状细胞mTOR/P70S6K信号通路的影响,以探讨肝复康抗纤维化的分子生物学机制。方法体外培养大鼠HSC-T6细胞,用肝复康对乙醛刺激的HSCs进行干预,采用RT-PCR法测定HSCs中Ⅰ、Ⅲ型胶原、mTOR、P70S6K的表达。结果空白对照组肝星状细胞各指标表达量呈相对较低水平,乙醛刺激组细胞Ⅰ型胶原、mTOR、P70S6K 和Ⅲ型胶原表达量明显升高（P<0.05）,而肝复康干预对于细胞Ⅰ型胶原、mTOR和P70S6K的升高有明显的抑制作用（P<0.01）。结论mTOR/P70S6K信号通路在HSCs活化过程中起到重要作用,肝复康发挥抗纤维化的作用机制与阻断mTOR/P70S6K信号通路有关。

关键词: 肝复康, 肝星状细胞, mTOR/P70S6K信号通路

Abstract: Objective To study the effect of blood serum supplemented with Ganfukang compound,a traditional Chinese herb medicine,on the mTOR/P70S6K signal pathway in hepatic stellate cells（HSCs）.Methods HSC-T6 stimulated by acetaldehyde were incubated with blood serum of Ganfukang compound.The expressions of collagen-Ⅰ,Ⅲ,mTOR and P70S6K in each group were analyzed by RT-PCR. Results HSCs exhibited a low mRNA level of collagen-Ⅰ,Ⅲ,mTOR and P70S6K in the control group,whereas they were remarkably increased in the model group（P<0.01 or P<0.05）,and the expression of them were downregulated after treated with blood serum of Ganfukang compound（P<0.01）.Conclusions mTOR/P70S6K signal pathway play an important roles in HSCs activation,and the anti-fibrosis effects of Ganfukang are related to the inhibition of mTOR/P70S6K signal pathway.

Key words: Hepatic stellate cells, Ganfukang, mTOR/P70S6K signal pathway

张坤, 姜妙娜, 张彩华, 贾玉杰. 中药肝复康对肝星状细胞mTOR/P70S6K信号通路的干预作用[J]. 实用肝脏病杂志, 2012, 15(1): 24-25.

Zhang Kun, Jiang Miaona, Zhang Caihua, et al. Effect of Chinese traditional medicine Ganfukang on the mTOR/P70S6K signal pathway in liver stellate cells[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2012, 15(1): 24-25.

参考文献

[1] Wynn TA.Cellular and molecular mechanisms of fibrosis.J Pathol,2008,214（2）:199-210.
[2] Gressner AM,Weiskirchen R. Modern pathogenetic concepts of liver fibrosis suggest stellate cells and TGF-beta as major players and therapeutic targets. J Cell Mol Med,2006,10（1）:76-99.
[3] Zheng Y,Collins SL,Lutz MA,et al.A role for mammalian target of rapamycin in regulating T cell activation versus anergy.J Immunol,2007,178:2163-2170.
[4] Patsenker E.Potent antifibrotic activity of mTOR inhibitors sirolimus and everolimus but not of cyclosporine A and tacrolimus in experimental liver fibrosis. J Hepatol,2011,doi:10.1016/j.jhep.2010.10.044.
[5] 彭贵主,吴波,单人锋,等. mTOR/P70S6K信号通路在肝细胞肝癌中的表达及临床意义.世界华人消化杂志,2008,16（29）:3279-3282.
[6] 杨妙芳,许小兵,吴文娟,等. 核糖体S6激酶对肝星状细胞内胶原表达的调控. 第四军医大学学报,2008,29（19）:1753-1756.
[7] 车颖,徐婷婷,姜妙娜,等. 肝复康胶囊对实验性肝纤维化大鼠肝细胞的保护作用. 中药材,2004,27（6）:428-429.
[8] 车颖,徐婷婷,姜妙娜,等.肝复康抗大鼠肝纤维化的实验研究.中国中药杂志,2004,29（6）:599-600.
[9] Xu TT,Jiang MN,Li C.Effect of Chinese traditional compound,
Gan-fu-kang,on CCl4-induced liver fibrosis in rats and its probable molecular mechanisms.Hepatol Res,2007,37:221-229.
[10] Lou JL,Jiang MN,Li C,et al. Herb medicine Gan-fu-kang attenuates liver injury in a rat fibrotic model. J Ethnopharmacol,2010,128:131-138.
[11] 张彩华,崔晓栋,姜妙娜,等. 肝复康不同浓度含药血清对肝星状细胞Ⅰ、Ⅲ型胶原及细胞基质的影响. 中国组织工程研究与临床康复,2008,12（33）:6496-6499.
[12] Sonoyama T,Tamura N,Miyashita K,et al.Inhibition of hepatic damage and liver fibrosis by brain natriuretic peptide. FEBS Lett,2009,583（12）:2067-2070.
[13] Rao RD,Buekner JC,Sarkaria JN.Mammalian target of rapamyein（mTOR） inhibitors as anticancer agents.Curr Caneer Drug Targets,2004:4:621-635.
[14] Buck M,Chojkier M A.Ribosomal S-6 Kinase-mediated signal to C/EBP-b is critical for the development of liver fibrosis.PLoS ONE,2007,2（12）:e1372.
[15] Jamison JT,Kayali F,Rudolph J,et al.Persistent redistribution of poly- adenylated mRNAs correlates with translation arrest and cell death following global brain ischemia and reperfusion.Neuroseience,2008,154:504-520.

中药肝复康对肝星状细胞mTOR/P70S6K信号通路的干预作用

Effect of Chinese traditional medicine Ganfukang on the mTOR/P70S6K signal pathway in liver stellate cells

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	周丽云, 李校天, 李丽, 杨俊超. 1,25（OH）₂D₃通过调节miR-146a水平抑制大鼠肝纤维化的体内和体外观察[J]. 实用肝脏病杂志, 2019, 22(3): 333-336.
[2]	潘高峰综述, 傅茂英, 郜玉峰审校. 内质网应激与肝纤维化关系研究进展^*[J]. 实用肝脏病杂志, 2018, 21(6): 987-990.
[3]	秦雨萌, 陈胜华. 肝纤维化进程中细胞外基质对肝星状细胞的作用及机制研究进展^*[J]. 实用肝脏病杂志, 2017, 20(3): 381-384.
[4]	张坤，胡海，张圆，刘佳. 大鼠肝星状细胞CTGF、纤维连接蛋白和整合素的表达变化及肝复康的调节作用*[J]. 实用肝脏病杂志, 2017, 20(2): 153-156.
[5]	仝艳艳, 李光明, 邓怡林, 石翠翠, 黄福德, 范建高. 氧化苯砷体外对大鼠原代肝星状细胞活化的阻抑作用研究^*[J]. 实用肝脏病杂志, 2016, 19(4): 413-417.
[6]	施凤综述, 王农荣审校. 促纤维化因子在肝纤维化发生中的作用研究进展[J]. 实用肝脏病杂志, 2016, 19(4): 505-508.
[7]	胡烨, 张国. 肝纤维化的药物治疗研究进展*[J]. 实用肝脏病杂志, 2016, 19(1): 8-11.
[8]	葛文松，范建高，李光明. 小分子干扰RNA沉默高迁移率族蛋白B1抑制肝星状细胞Ⅰ型和Ⅲ型胶原合成^*[J]. 实用肝脏病杂志, 2015, 18(4): 340-343.
[9]	王军，李光明，仝艳艳，邓怡林，吴劼，范建高. 新型异喹啉类化合物12-4-Y对活化肝星状细胞凋亡的影响及其机制研究^*[J]. 实用肝脏病杂志, 2015, 18(4): 344-347.
[10]	江远, 张玲, 钟肖英, 吴文如, 何金洋. 铁沉积对大鼠肝纤维化影响的机制研究^*[J]. 实用肝脏病杂志, 2015, 18(2): 173-177.
[11]	李欣, 朱英. 肝纤维化发生相关信号传导通路研究进展^*[J]. 实用肝脏病杂志, 2015, 18(1): 101-104.
[12]	郝书理, 张纪元, 金磊, 孙颖, 李保森, 冯国华, 王福生, 邹正升. 酒精性肝病患者血浆白细胞介素-21水平及重组IL-21对LX-2肝星状细胞增殖和活化的影响^*[J]. 实用肝脏病杂志, 2014, 17(1): 18-21.
[13]	彭梅娟, 郝春秋, 张野, 谢玉梅, 张岩, 白雪帆. 抑制结缔组织生长因子表达对肝星状细胞增殖和活化的影响^*[J]. 实用肝脏病杂志, 2012, 15(5): 430-433.
[14]	张坤, 姜妙娜, 张彩华, 贾玉杰. 肝复康对肝纤维化大鼠肝组织整合素α5β1/FAK信号通路的调节作用^*[J]. 实用肝脏病杂志, 2012, 15(2): 104-107.
[15]	李骢, 雷鸿雁, 姜妙娜, 张彩华, 贾玉杰. 肝纤维化大鼠肝组织Raf/ERK信号通路的变化及肝复康的干预作用^*[J]. 实用肝脏病杂志, 2012, 15(2): 108-110.