乙型肝炎肝硬化患者外周血树突状细胞亚群的鉴定及其临床意义

doi:10.3969/j.issn.1672-5069.2014.03.014

摘要/Abstract

摘要： 目的探讨检测乙型肝炎肝硬化患者外周血树突状细胞（DC）亚群的临床意义。方法采用流式细胞分析技术检测42例乙型肝炎肝硬化患者、45例慢性乙型肝炎患者和30例健康人外周血DC细胞亚群和淋巴细胞亚群的构成,比较三者间的差异。结果乙型肝炎肝硬化患者外周血DC1和DC2百分数分别为（0.2±0.1）%和（0.2±0.04）%,慢性乙型肝炎患者为（0.3±0.1）%和（0.2±0.1）%,均显著低于健康人群[（0.4±0.1）%和（0.3±0.1）%,P<0.01];13例HBeAg阳性乙型肝炎肝硬化患者外周血DC2亚群细胞绝对计数为（5.62±1.28）个/μL,显著低于29例HBeAg阴性的乙型肝炎肝硬化患者[（8.75±2.32）个/μL,P<0.05],但是两者DC1细胞水平无明显差别;乙型肝炎肝硬化患者外周血CD4⁺T淋巴细胞和CD8⁺T淋巴细胞计数分别为（588.4±124.2）个/μL和（338.5±97.4）个/μL,均显著低于慢性乙型肝炎患者[（686.7±106.5）个/μL和（432.1±102.6）个/μL,P<0.05],而且乙型肝炎肝硬化患者和慢性乙型肝炎患者外周血CD8⁺T细胞数均显著低于健康对照人群[（560.2±105.6）个/μL,P<0.05]。结论乙型肝炎肝硬化患者外周血DC1和DC2数量减少,伴CD8⁺T细胞数降低,可能是导致肝硬化形成的原因之一。

关键词: 肝硬化, 慢性乙型肝炎, 树突状细胞, 外周血

Abstract: Objective To evaluate the clinical implication of peripheral blood dendritic cell （DC） subsets in cirrhotic patients with hepatitis B virus （HBV） infection. Methods Flow cytometry was used to analyze the counts of peripheral blood DC subsets and T lymphocyte subsets in 42 cirrhotic patients with HBV infection,45 patients with chronic hepatitis B and 30 healthy persons. Results The percentages of DC1 and DC2 subsets were （0.2±0.1）% and（0.2±0.04）%,respectively,in cirrhotic patients with chronic HBV infection, and （0.3±0.1）% and （0.2±0.1）%,respectively,in patients with chronic hepatitis B,all were significantly lower than those in health controls [（0.4±0.1）% and （0.3±0.1）%,P<0.01]; The DC2 subset in 13 cirrhotic patients with HBeAg-positive was （5.62±1.28） cells per microliter, significantly lower than that in 29 cirrhotic patients with HBeAg-negtive[（8.75±2.32）cells per microliter,P<0.05];No significant difference was found in DC1 subsets between cirrhotic patients with HBeAg-positive and HBeAg-negative;The CD4⁺ T lymphocyte and CD8⁺ T lymphocyte counts in cirrhotic patients were（588.4±124.2） cells per microliter and （338.5±97.4）cells per microliter,both significantly lower than those in patients with chronic hepatitis B[（686.7±106.5） cells per microliter and （432.1±102.6）cells per microliter,P<0.05];Moreover,CD8⁺ T lymphocytes in patients with cirrhosis and with chronic hepatitis B were both significantly lower than that in health controls[（560.2±105.6） cells per microliter,P<0.05]. Conclusion The decrease in DC1 and DC2 subsets in cirrhotic patients with HBV infection,accompanied with a decrease in CD8⁺ T lymphocytes,might play an important role in the development of cirrhosis after HBV infection.

Key words: Cirrhosis, Hepatitis B, Dendritic cell, Peripheral blood

郑盛, 唐映梅, 杨晋辉. 乙型肝炎肝硬化患者外周血树突状细胞亚群的鉴定及其临床意义[J]. 实用肝脏病杂志, 2014, 17(3): 275-279.

Zheng Sheng, Tang Yingmei, Yang Jinhui. Clinical implication of peripheral blood dendritic cell subsets in cirrhotic patients with hepatitis B virus infection[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2014, 17(3): 275-279.

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