肝复康对大鼠肝组织血小板衍生生长因子表达及对胶原合成的影响*

doi:10.3969/j.issn.1672-5069.2012.02.010

实用肝脏病杂志 ›› 2012, Vol. 15 ›› Issue (2): 111-113.doi: 10.3969/j.issn.1672-5069.2012.02.010

肝复康对大鼠肝组织血小板衍生生长因子表达及对胶原合成的影响^*

李骢, 周情, 姜妙娜, 张彩华, 贾玉杰

116044 辽宁省大连市大连医科大学病理生理学教研室（李骢,姜妙娜,张彩华,贾玉杰）;
山东能源淄矿集团中心医院（周情）

收稿日期:2012-02-15 出版日期:2012-04-10 发布日期:2017-03-07
通讯作者: 贾玉杰,E-mail:pathophy@163.com
作者简介:李骢女,44岁,医学博士,教授。主要从事消化系统疾病的基础研究。E-mail:goodluck_licong@163.com
基金资助:
辽宁省自然科学基金（编号：201102055）

The influence of Gan-fu-kang on hepatic PDGF expression in rats with hepatic fibrosis

Li Cong, Zhou Qing, Jiang Miaona, et al.

Department of Pathophysiology,Dalian Medical University,Dalian 116044,Liaoning Province,China

Received:2012-02-15 Online:2012-04-10 Published:2017-03-07

摘要/Abstract

摘要： 目的探讨中药肝复康对大鼠肝组织血小板衍生生长因子（PDGF）表达的影响及其对胶原合成的调节作用。方法制备四氯化碳诱导的肝纤维化大鼠模型,应用肝复康干预;测定各组大鼠血清白蛋白、球蛋白、谷丙转氨酶和谷草转氨酶的含量;采用免疫组织化学法检测肝组织PDGF表达;采用RT-PCR法测定肝组织及HSC-T6细胞Ⅰ型胶原mRNA水平的变化。结果治疗组与模型组相比,血清谷丙转氨酶、谷草转氨酶和球蛋白水平均降低,白蛋白及白/球比例则升高（P<0.01）;模型组肝组织PDGF的表达上调,而肝复康治疗组表达则下调（P<0.01）;模型组肝组织及HSC-T6细胞Ⅰ型胶原mRNA水平较正常对照组明显上调（P<0.01）,而肝复康治疗组则明显下调（P<0.01）。结论肝复康通过降低PDGF的表达,抑制Ⅰ型胶原的合成,对肝纤维化具有一定的防治作用。

关键词: 肝纤维化, 肝星状细胞, 肝复康, 血小板衍生生长因子, Ⅰ型胶原

Abstract: Objective To investigate the influence of Gan-fu-kang on hepatic PDGF expression in rats with hepatic fibrosis. Methods The rats with liver fibrosis were made by subcutaneously injection of 10%CCl₄ and treated with Gan-fu-kang,a herbal medicine. Serum albumin,globulin,ALT and AST were measured. The PDGF expression in liver tissues were detected by immunohistochemistry staining. The collagenⅠmRNA were detected by RT-PCR. Results Compared with in model group,the levels of serum ALT,AST and globulin decreased,and albumin and the ratio of albumin to globulin increased （P<0.01） in Gan-fu-kang treatment group; the expression of PDGF in liver tissues in model group was obviously elevated than in Gan-fu-kang treatment group（P<0.01）. the collagenⅠmRNA level in model group increased than in control group and in treatment group（P<0.01）. Conclusion Gan-fu-kang has some effects on liver fibrosis by decreasing PDGF expression and inhibiting collagenⅠsynthesis.

Key words: Liver fibrosis, Hepatic stellate cell, Gan-fu-kang, PDGF, Collagen Ⅰ

李骢, 周情, 姜妙娜, 张彩华, 贾玉杰. 肝复康对大鼠肝组织血小板衍生生长因子表达及对胶原合成的影响^*[J]. 实用肝脏病杂志, 2012, 15(2): 111-113.

Li Cong, Zhou Qing, Jiang Miaona, et al.. The influence of Gan-fu-kang on hepatic PDGF expression in rats with hepatic fibrosis[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2012, 15(2): 111-113.

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肝复康对大鼠肝组织血小板衍生生长因子表达及对胶原合成的影响^*

The influence of Gan-fu-kang on hepatic PDGF expression in rats with hepatic fibrosis

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