急性肝衰竭大鼠胃肠运动改变与肠神经元关系研究*

doi:10.3969/j.issn.1672-5069.2011.01.002

Abstract

Abstract: Objective This study was undertaken to explore the relationship between enteric neuronal and migrating motor complexes（MMC）in rats with acute liver failure. Methods Wistar rats were given the D-galactosamine hydrochloride（D-Gal）to induce acute liver failure. Then，the MMC was recorded by a multilead physiologival recording system. TUNEL，5-HT and NSE immunohistochemistry were used to detect neuronal apoptosis. Results The duration of jejunal MMC was significantly prolonged in rats with acute liver failure compared with the control（P<0.05）；The duration of phaseⅡ was also significantly prolonged，and multiple clusters of contractions was observed in phaseⅡ；However，the duration of phaseⅢ was significantly shortened（P<0.05）compared with the control group；TUNEL staining showed neuronal apoptosis increased significantly in rats with acute liver failure；The content of NSE and 5-HT positive substances of jujunal in rats with acute liver failure was markedly decreased. Conclusion The prolonged duration of MMC and phaseⅡ and decreased duration of phaseⅢ might results in abnormal gastrointestinal motility，which may be related to enteric neuronal apoptosis.

Key words: Acute liver failure, Migrating motor complex, Enteric neuronal

XU Weihong，LIU Mei，LV Zhiwu，et al.. The relationship between enteric neuronal and gastrointestinal motility in rats with acute liver failure[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2011, 14(1): 3-6.

References

[1] JOHN A，SUMEDHA G，JENNIFER Y，et al. Gut flora and bacterial translocation in chronic liver disease[J]. World J Gastroenterol，2006，12（10）:1493-1502.
[2] ANDREWS JM，DONOVAN DG，HEBBARD GS，et al. Human duodenal phase Ⅲ migrating motor complex activity is predominantly antegrade as revealed by high-resolution manometry and colour pressure plots[J]. Neurogastroenterol Motil，2002，14（4）:331-338.
[3] YOQO K，OZAKI K，TAKANASKI H，et al. Effects of motilin and mitemcinal（GM-611）on gastrointestinal activity in rhesus monkeys in vivo and in vitro[J]. Dig Dis Sci，2007，52（11）:3112-3122.
[4] DEPOORTERE I，DEWINTER B，THIJS T，et al. Comparison of the gastroprokinetic effects of ghrelin，GHRP-6 and motilin in rats in vivo and in vitro[J]. Eur J pharmacol，2005，515:160-168.
[5] SEAN M，GERALD J，KENTON M，et al. Interstitial cells of Cajal in the deep muscular plexus mediate enteric motor neurotransmission in the mouse small intestine[J]. J Physiol，2006，573（1）:147-159.
[6] NICK J，SPENCE R，GRANT W，et al. Synchronization of enteric neuronal firing during the murine colonic MMC[J]. J Physiol，2005，564（3）：829-847.
[7] YOUNG HM. Functional development of the enteric nervous system--from migration to motility[J]. Neurogastroenterol Motil，2008，20（Suppl 1）:20-31.
[8] ROMASKI KW. Characteristics of phase3-like activity and rebound excitation triggered by hexamethonium and atropine administration in the ovine small bowel[J]. Indian J Exp Biol，2010，48（2）:124-132.
[9] EAMONN JD，DANTE JH，CONOR J，et al. The mechanisms underlying the generation of the colonic migrating motor complex in both wild-type and nNOS knockout mice[J]. Am J Physiol Gastrointest Liver Physiol，2010，298（3）:222-232.
[10] SPENCER NJ，HENNIG GW，DICKSON E，et al. Synchronization of enteric neuronal firing during the murine colonic MMC[J]. J Physiol，2005，564（3）:829-847.
[11] NGUYEN NQ，FRASER R，CHAPMAN M. et al. Relationship between intragastric meal distribution and delayed gastric emptying in critical illness[J]. Neurogastroenterol Motil，2006，18（8）:744.
[12] HEREDIA DJ，DICKSON EJ，BAYGUINOV PO，et al. Localized release of serotonin（5-hydroxytryptamine）by a fecal pellet regulates migrating motor complexes in murine colon[J]. Gastroenterology，2009，136（4）:1328-1338.

The relationship between enteric neuronal and gastrointestinal motility in rats with acute liver failure

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 9

Recommended Articles

Metrics

Comments

[1]	Zhang Xuan, Tang Xiaoyun, Tang Chunyin, et al. Effect of Chinese herbal medicine on intestinal microflora in mice with D-galactosamine/LPS-induced acute liver failure [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2019, 22(4): 478-481.
[2]	Deng Yilin, Yu Heguo, Shi Min, et al.. Suppression of TNF-α and JNK-related pro-apoptotic signaling expression of oridonin in mice with LPS/D-Gal-induced acute liver failure [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2017, 20(4): 397-401.
[3]	Deng Yilin, Yu Heguo, Shi Min, et al.. Effect of oridonin on hepatic cytokine levels in mice with LPS/D-Gal-induced acute liver failure [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2017, 20(3): 294-297.
[4]	Li Shuang, Chen Yu. You'an.. Severity evaluation of acute liver failure [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2017, 20(1): 17-20.
[5]	Liu Ziyun,Tian Deying,Wu Liang. Changes of IL-25 and IL-17RB mRNA in mice with D-galactosamine and lipopolysaccharide-induced acute liver failure [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2016, 19(3): 275-278.
[6]	Cao Haixia, Fan Jiangao. What is special in patients with Wilson's disease in children and pregnant women [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2015, 18(1): 89-91.
[7]	Wang Bo, Tang Xiaopeng. Experimental study of transplantations of umbilical cord blood stem cells combined with bone marrow stem cells for rats with acute liver failure [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2014, 17(1): 58-63.
[8]	Zhao Pan, Bi Zhenhua, Wang Chunya. Serum inflammatory cytokines levels in patients with acute liver failure [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2014, 17(1): 63-65.
[9]	PAN Qiaoling，LIU Xiaojing，CHEN Tianyan.. Correlation of the plasma levels of neurotensin and calcitonin gene-related peptide to the severity of hepatic encephalopathy in rats with acute liver failure [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2010, 13(6): 404-406.