脐血干细胞与骨髓干细胞联合移植治疗大鼠急性肝衰竭实验研究

doi:10.3969/j.issn.1672-5069.2014.01.017

Abstract

Abstract: Objective To observe the therapeutic effects of co-transplanted umbilical cord blood stem cells and bone marrow stem cells for rats with acute liver failure induced by D-galactose. Methods Mononuclear cells isolated from umbilical cord blood and bone marrow of rats were cultured in medium containing hepatocyte growth factor（HGF） and stem cell factor（SCF） for 3 weeks,and the expression of hepatocyte markers,such as AFP and ALB,were detected by immunocytochemistry;A rat model of acute liver failure was established by D-galactose injection（1.4 g.kg^-1,intraperitoneally） for 24 h. Rats with acute liver failure were subjected to transplantation of bone marrow stem cells,umbilical cord blood stem cells,and umbilical cord blood stem cell mixed with bone marrow stem cell,or an equal amount of saline per day for seven days. The survival rates,liver function and pathological changes in liver were studied. Results Bone marrow stem cells and umbilical cord blood stem cells were successfully proliferated and differentiated into hepatocytes in vitro by stimulation of HGF and SCF. The 9-day survival rates of rats with acute liver failure in umbilical cord blood stem cell,bone marrow stem cell and mixed stem cell transplantation group were 55.6%,50.0% and 77.8%,respectively,all of which were markedly higher than that in rats treated with saline（16.7%,P<0.01）;The 9-day survival rate of rats in mixed cell transplantation group was significantly higher than that of rats in umbilical cord blood stem cell or bone marrow stem cell alone （P<0.01）. Conclusions Bone marrow stem cell and umbilical cord blood stem cell transplantation improve liver function of rats with acute liver failure,and combinational transplantation of these two cells is efficient.

Key words: Acute liver failure, Stem cell, Cell transplantation, Bone marrow stem cell, Umbilical cord blood stem cell, Rats

Wang Bo, Tang Xiaopeng. Experimental study of transplantations of umbilical cord blood stem cells combined with bone marrow stem cells for rats with acute liver failure[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2014, 17(1): 58-63.

References

[1] Laurson J,Selden C,Hodgson HJ. Hepatocyte progenitors in man and in rodents—multiple path ways,multiple candidates. Int J Exp Pathol,2005,86（1）：1-18.
[2] Navarro-Montero O,Romero-Moya D,Montes R,et al. Intrahepatic transplantation of cord blood CD34 + cells into newborn NOD/SCID-IL2Rγ（null） mice allows efficient multi-organ and multi-lineage hematopoietic engraftment without accessory cells. Clin Immunol,2012,145（2）：89-91.
[3] Kakinuma S,Tanaka Y,Chinzei R,et al. Human umbilical cord blood as a source of transplantable hepatic progenitor cells. Stem Cells,2003,21：217-227.
[4] Tang XP,Yang X,Tan H,et al. Clinical and experimental study on therapeutic effect of umbilical cord blood transplantation on severe viral hepatitis. World J Gastroenterol,2003,9：1999-2003.
[5] Lee HJ,Jung J,Cho KJ,et al. Comparison of in vitro hepatogenic differentiation potential between various placenta-derived stem cells and other adult stem cells as an alternative source of functional hepatocytes. Differentiation,2012,84（3）：223-231.
[6] Aurich I,Mueller LP,Aurich H,et al. Functional integration of hepatocytes derived from human mesenchymal stem cells into mouse livers. Gut,2007,56（3）：405-415.
[7] Danet GH,Luongo JL,Bulter G,et al. CiqRp defines a new human stem cell population with hematopoitic and hepatic potential. Proc Natl Acad Sci USA,2002,99（16）：10441-10445.
[8] Alison MR,Poulsom R,Jeffery R,et al. Hepatoeytes from nonhepatie adult stem cells. Nature,2000,406：257-261.
[9] Theise ND,Nimmakayalu M,Gardner R,et al. Liver from bone marrow in humans. Hepatology,2000,32（1）：11-16.
[10] Theise ND,Badve S,Saxane R,et al. Derivation of hepatocytes from bone marrow cells in mice after radiation induced myelablation. Hepatology,2000,31（1）：235-240.
[11] Kang XQ. Fibroblast growth factor-4 and hepatocyte growth factor induce differentiation of human umbilical cord blood-derived mesenchymal stem cells into hepatocytes. World J Gastroenterol, 2005,11（47）,7461-7465.
[12] 李文晰,段芳龄. 人骨髓单个核细胞向肝细胞诱导分化的体外研究. 胃肠病学和肝脏病学杂志,2004,12（2）：144-147.
[13] Oh SH,Miyazaki M,Kouchi H,et al. Hepatocyte growth factor induces differentiation of adult rat bone marrow cells into a hepatocyte lineage invitro. Biochem Biophys Res Commun,2000,279：500-504.
[14] Theise ND,Badve S,Saxena R,et al. Derivation of hepatocytes from bone morrow cells in mice after radiation-induced myeloablation. Hepatology,2000,31：235-224.
[15] 季凤清,王屹,孙海梅,等. 人脐血非造血干细胞免疫原性的实验研究. 解剖学报,2008,39（1）：55-59.

Experimental study of transplantations of umbilical cord blood stem cells combined with bone marrow stem cells for rats with acute liver failure

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Xu Fei, Li Weirong.. Protective effects of astragaloside in rats with CCL₄-induced liver injuries [J]. Journal of Practical Hepatology, 2019, 22(6): 808-811.
[2]	Gao Lei, Cao Lei, Wang Ruifang, et al.. Protective effect of human umbilical cord mesenchymal stem cell transplantation on alcoholic liver injury in rats [J]. Journal of Practical Hepatology, 2019, 22(6): 812-815.
[3]	Lin Qiuxiang, Wang Xuewen, Huang Zuxiong, et al. Protective effects of breviscapine on liver and intestinal mucosal barrier functions in rats with hepatic ischemia-reperfusion injury [J]. Journal of Practical Hepatology, 2019, 22(5): 636-639.
[4]	Shi Xiaolong, Du Rui, Qin Geping, et al. Protective effect of sevoflurane on sepsis-induced liver injury in rats [J]. Journal of Practical Hepatology, 2019, 22(5): 640-643.
[5]	Zhao Fangfang, Zhai Yongzhen, Feng Guohe. Efficacy and safety of autologous peripheral blood stem cell transplantation in patients with hepatitis B cirrhosis [J]. Journal of Practical Hepatology, 2019, 22(5): 700-703.
[6]	Wang Yan, Su Feng, Li Yuanyuan, et al. Protective effect of bee venom hemolysin on rats with nonalcoholic fatty liver disease [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2019, 22(4): 474-477.
[7]	Zhang Xuan, Tang Xiaoyun, Tang Chunyin, et al. Effect of Chinese herbal medicine on intestinal microflora in mice with D-galactosamine/LPS-induced acute liver failure [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2019, 22(4): 478-481.
[8]	Zhou Liyun, Li Xiaotian, Li Li. 1,25（OH）₂D₃ inhibits hepatic fibrosis by regulating miR-146a levels in vitro and in vivo [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2019, 22(3): 333-336.
[9]	Zhu Yun, Yao Genhong, Tang Xiaojun. Mesenchymal stem cell transplantation alleviating hepatic injury by modulating intrahepatic biliary epithelial cell autophagic flux in mice with 2-OA-BSA-induced primary biliary cholangitis [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2019, 22(3): 337-340.
[10]	Guo Liping, Zhang Chenghong, Ning Baoshuo, et al.. An improved approach for seperating hepatocytes from neonatal rats in vitro [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2019, 22(1): 17-20.
[11]	Yusup Tursun, Zhao Yanxia.. Establishment of low-dose diethylnitrosamine-induced liver fibrosis model in rats [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2019, 22(1): 33-36.
[12]	Yan Huanjie, Zhang Shuqin, Zhao Wenjing.. Therapeutic efficacy of autologous bone marrow stem cell transplantation for treatment of patients with hepatitis B-induced acute-on-chronic liver failure [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2019, 22(1): 81-84.
[13]	Wang Chan, Qin Wei, Dai Yong, et al.. Clinical efficacy of autologous peripheral blood stem cell transplantation in patients with hepatitis B cirrhosis [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2019, 22(1): 97-100.
[14]	Liu Enqiang, Chen Guo, Liao Xiuyong, et al.. Inhibition of TGF-β1/Smad/ERK passway by astaxanthin in rats with CCl₄-induced liver fibrosis [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2018, 21(6): 842-846.
[15]	Zhang Junfei, Song Haiyan, Chen Xi, et al.. Clinical observation of intrahepatic human umbilical cord mesenchymal stem cell transplantation in the treatment of patients with hepatitis B-induced decompensated liver cirrhosis [J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2018, 21(5): 693-696.