PAX6通过MEK/ERK信号通路抑制肝星状细胞活化和增殖实验研究

doi:10.3969/j.issn.1672-5069.2022.03.004

Abstract

Abstract: Objective The purpose of this experiment was to investigate the effect of PAX6 on inhibition of activation and proliferation of hepatic stellate cells (HSCs) by through MEK/ERK signaling pathway. Methods The LX2 HSCs were divided into control, PAX6 inhibitor and PAX6 mimics group and managed accordingly. The cell proliferation was determined by CCK-8 kit, the cell differentiation was detected by oil red O staining, the cell apoptosis was analyzed by flow cytometry, and PAX6, MEK and ERK mRNA and their protein expression were assayed by RT-PCR and Western blotting, respectively. Results The PAX6 mRNA level, apoptosis rate and G1 stage in PAX6 inhibitor-intervened group were (1.49±0.23), (2.70±0.85)% and (59.02±1.25)%, significantly lower than [(1.85± 0.19), (3.40±0.47)% and (64.66±1.41)%, respectively, P<0.05] in LX2 HSCs, and the cell proliferation, survival rate, differentiation rate, MEK and ERK mRNA and their protein expression in PAX6 inhibitor-intervened group were (0.79±0.03), (73.35±9.74)%, (49.37±4.24)%, (2.55±0.43), (3.90±0.49), (0.89±0.15) and (1.17±0.17), significantly higher than [(0.58±0.05), (60.74±9.24)%, (29.35±4.47)%, (2.67±0.47), (4.55±0.50), (0.74±0.14) and (1.35±0.16), respectively, P<0.05] in the control; the PAX6 mRNA level, apoptosis rate and G1 stages in PAX6 mimics-intervened group were (2.67±0.20), (6.70±1.04)% and (66.38±1.35)%, significantly higher than [(1.85± 0.19), (3.40±0.47)% and (64.66±1.41)%, respectively, P<0.05] in the control, and the cell proliferation, survival rate, differentiation rate, MEK and ERK mRNA and their protein expression in PAX6 mimics group were (0.40±0.04), (52.24±5.57)%, (13.85±3.35)%, (1.94±0.53), (1.45±0.42), (0.53±0.15) and (0.53±0.16), significantly lower than [(0.58 ±0.05), (60.74±9.24)%, (29.35±4.47)%, (2.67±0.47), (4.55±0.50), (0.74±0.14) and (1.35±0.16), respectively, P<0.05] in the control. Conclusion The PAX6 overexpression could inhibit the activation and proliferation of hepatic stellate cells, and promote cell apoptosis. The mechanism involved might be related to the inhibition of MEK and ERK expression and MEK/ERK pathway in hepatic stellate cells.

Key words: Hepatic stellate cells, PAX6, MEK/ERK signaling pathway, Cell activation, Cell proliferation, In vitro

Bai Liang, Wang Baotai, Gao Zhifeng, et al. PAX6 inhibiting activation and proliferation of hepatic stellate cells through MEK/ERK signaling pathway in vitro[J]. Journal of Practical Hepatology, 2022, 25(3): 318-322.

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PAX6 inhibiting activation and proliferation of hepatic stellate cells through MEK/ERK signaling pathway in vitro

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