人EFTUD2基因靶向小分子化合物筛选细胞模型的建立*

doi:10.3969/j.issn.1672-5069.2021.04.005

Abstract

Abstract: Objective This paper aimed to establish a cell model with luciferase as reporter gene for screening human EFTUD2 gene-targeting compounds. Methods After double-digestion of LV6 lentivirus vector by restrictive enzymes, the target fragment was recombined into the LV6 lentivirus vector.The HepG2 cells were further infected with the recombinant lentivirus, and the Epro-LUC-HepG2 cell line was obtained by puromycin screening and limited dilution. By using the luciferase reporter gene system, we select the best single clone cell lines and expand the culture. Results The LV6-Epro0.5-LUC lentivirus vector was successfully constructed, and the Epro-LUC-HepG2 monoclonal cell lines expressing luciferase was successfully screened by lentivirus transfection and puromycin selection. According to the results of luciferase reporter gene detection, the best monoclonal cells were selected and named as Epro-LUC-HepG2 cells. Conclusion The Epro-LUC-HepG2 cells, a compounds screening model of targeting human EFTUD2 gene, is successfully constructed, which laid a hope for further screening of new targeted immunoregulatory agents.

Key words: Cell model, EFTUD2, Lentivirus vector, Drug screening, In vitro

Tian Anran, Xu Ruirui, Hu Pingping, et al. Establishment of a cell model for screening human EFTUD2 gene-targeting small molecule compounds[J]. Journal of Practical Hepatology, 2021, 24(4): 472-475.

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Establishment of a cell model for screening human EFTUD2 gene-targeting small molecule compounds

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