c-Met基因过表达脐带间充质干细胞株的构建*

doi:10.3969/j.issn.1672-5069.2018.03.020

Abstract

Abstract: Objective To construct the overexpression of c-met in human umbilical cord mesenchymal stem cells（hUMSCs） with lenti-vector transfection in vitro. Methods The phenotype of hUMSCs was determined by Flow cytometry analysis, and the hUMSCs were transfected by different multiple of infection（MOI with c-met lenti-vector. The optimal MOI was determined under fluorescence microscopy. The c-Met protein was detected by Western blot. Results P5 gegeration of hUMSCs were CD44/CD90/CD105-positive,and CD31/CD45/CD34-negative, indicating the hUMSCs were obtained with specific surface markers. The optimal MOI was 80, and after puromycin screening,the transfected efficiency was up to 100%. The c-Met protein was overexpressed in the transfected hUMSCs by Western blot detection. Conclusion c-Met overexpressing hUMSCs were successfully constructed, which might be beneficial for further study.

Key words: Human umbilical cord mesenchymal stem cells, c-Met, Transduction, In vitro

Zhang Yongting, Zhu Chuanlong, Li Jun, et al. Construction of overexpression of c-met in human umbilical cord mesenchymal stem cells with lenti-vector transfection in vitro[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2018, 21(3): 397-400.

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Construction of overexpression of c-met in human umbilical cord mesenchymal stem cells with lenti-vector transfection in vitro

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