葡萄糖神经酰胺葡萄糖基转移酶siRNA对7702肝细胞增殖的影响及机制研究*

doi:10.3969/j.issn.1672-5069.2018.05.011

Abstract

Abstract: Objective To investigate the impact of UGCG siRNA on 7702 hepatocyte proliferation in vitro. Methods The 7702 hepatocytes were cultured in vitro and UDP-glucose ceramide glucosyltransferase（UGCG） siRNA was transfected into the hepatocytes. MTT was performed to detect the cell proliferation,the Bcl-2,Bax,Caspase 3 gene were detected by real-time quantitative PCR,and the expression of Caspase 3 protein in hepatocytes was detected by Western blot. Results UGCG siRNA successfully down-regulated glucosylceramide synthase mRNA levels as compared to that in the control（P<; 0.05）;the proliferation of hepatocytes was inhibited（P<; 0.05） after transfection of the glycosylated ceramide synthase gene,Bcl-2 mRNA decreased（P<; 0.05）,Bax mRNA increased （P<; 0.05）,and the expression of Caspase 3 protein was significantly upregulated（P<; 0.05）. Conclusion The changes of sphingolipid metabolism caused by the lack of glucosylceramide synthase is involved in hepatocyte proliferation, which might be related to the regulation of BCL2/BAX signal pathway.

Key words: 7702 hepatocytes, Sphingolipid, Cell proliferation, Glucosylceramide synthase, In vitro

Li Junfeng, Zheng Sujun, Liu Shuang, et al.. Impact of UGCG siRNA on 7702 hepatocyte proliferation in vitro[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2018, 21(5): 697-700.

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Impact of UGCG siRNA on 7702 hepatocyte proliferation in vitro

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