过表达富组氨酸糖蛋白质粒在体外抑制MHCC-97H肝癌细胞增殖

doi:10.3969/j.issn.1672-5069.2014.06.018

Abstract

Abstract: Objective To investigate the expression of histidine-rich glycoprotein（HRG） in hepatocellular carcinoma（HCC） and its impact on HCC cell proliferation. Methods Immunohistochemistry was used to detect the expression of HRG in 8 HCC specimens and Western blot to evaluate the expression of HRG in 11 surgical cancerous and its non-cancerous specimens. The cell proliferation was analyzed by flow cytometry after stable overexpression of HRG in MHCC-97H cells. Results Declined expression of HRG was found in all 8 cancerous tissues as compared to the non-tumor tissues;Similar results were obtained in 11 pairs of HCC and its para-carcinoma tissues;The relative expression of HRG/β-actin was（1.39±2.08） in para-carcinoma tissues, and （0.14 ± 0.17） in tumor tissue,with a significant difference between them（P<0.05）;The cell proliferation of MHCC-97H cells overexpressing HRG was remarkably inhibited as compared with the mock cells[（1.72±1.19）% vs.（14.3± 2.99）%,P<0.01]. Conclusion HRG is down-regulated in HCC and HRG overexpression can inhibit HCC cell proliferation in vitro.

Key words: Hepatocellular carcinoma, Histidine-rich glycoprotein, Cell proliferation, In vitro

Hu Xiaoyun, Chen Jinzhang, Liao Yujing. Overexpression of histidine-rich glycoprotein plasmids inhibits cell proliferation of MHCC-97H cells in vitro[J]. JOURNAL OF PRACTICAL HEPATOLOGY, 2014, 17(6): 632-636.

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Overexpression of histidine-rich glycoprotein plasmids inhibits cell proliferation of MHCC-97H cells in vitro

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