移植NAFLD患者和健康人粪菌对高脂饮食大鼠肠道菌群的影响*

doi:10.3969/j.issn.1672-5069.2020.01.036

Abstract

Abstract: Objective The aim of this study was to investigate the effects of fecal bacteria transplantation (FBT) on microflora in rats with high-fat-induced liver steatosis. Methods 32 rats were randomly divided into group A, B, C and D. The non-alcoholic fatty liver disease (NAFLD) model was established by high-fat feeding, and fecal bacteria transplantation from patients with NAFLD and healthy persons were performed. The 16 sDNA flora of intestinal mucosal tissue were analyzed. Results The content of Rodentibacter in the model group was increased as compared to that in the control, but it increased more greatly in the rats with FBT from NAFLD, and it decreased greatly in rats with FBT from healthy persons. Conclusion The bacteria flora of patients with NAFLD might be a risk factor for hepatic steatosis, and FBT by using healthy person’s feces might alleviate the liver steatosis.

Key words: Nonalcoholic fatty liver disease, Intestinal flora, Fecal bacteria transplantation, Abundance of flora, Rats

Luo Qingling, Zhou Yongjian, Tang Wenjuan, et al. Effects of fecal bacteria transplantation on microflora in rats with high-fat-induced liver steatosis[J]. Journal of Practical Hepatology, 2020, 23(1): 134-137.

References

[1] Younossi Z M, Koenig A B, Abdelatif D, et al. Global epidemiology of nonalcoholic fatty liver disease-Meta-analytic assessment of prevalence, incidence, and outcomes. Hepatology,2016,64(1):73-84.
[2] 池肇春. 非酒精性脂肪性肝炎肝硬化发病机制研究进展与展望. 实用肝脏病杂志,2018,21(2):166-169.
[3] Ridaura V K, Faith J J, Rey F E, et al. Gut microbiota from twins discordant for obesity modulate metabolism in mice. Science,2013,341(6150):1241214.
[4] De Palma G, Lynch M D J, Lu J, et al. Transplantation of fecal microbiota from patients with irritable bowel syndrome alters gut function and behavior in recipient mice. Sci Translat Med,2017,9(379):f6397.
[5] Li B, Guo K, Zeng L, et al. Metabolite identification in fecal microbiota transplantation mouse livers and combined proteomics with chronic unpredictive mild stress mouse livers. Transl Psychiat,2018,8(1):34.
[6] Wong S H, Zhao L, Zhang X, et al. Gavage of fecal samples from patients with colorectal cancer promotes intestinal carcinogenesis in germ-free and conventional mice. Gastroenterology,2017,153(6):1621-1633.
[7] Rabot S, Membrez M, Bruneau A, et al. Germ-free C57BL/6J mice are resistant to high-fat-diet-induced insulin resistance and have altered cholesterol metabolism. FASEB J,2010,24(12):4948-4959.
[8] Zeng H, Liu J, Jackson M I, et al. Fatty liver accompanies an increase in lactobacillus species in the hind gut of C57BL/6 mice fed a high-Fat diet. J Nutrit,2013,143(5):627-631.
[9] Zhou D, Pan Q, Xin F Z, et al. Sodium butyrate attenuates high-fat diet-induced steatohepatitis in mice by improving gut microbiota and gastrointestinal barrier. World J Gastroenterol,2017,23(1):60-75.
[10] Schramm C. Bile acids, the microbiome, immunity, and liver tumors. New Engl J Med,2018,379(9):888-890.
[11] Cheng S L, Li X, Lehmler H J, et al. Gut microbiota modulates interactions between polychlorinated biphenyls and bile acid homeostasis. Toxicol Sci,2018,166(2):269-287.
[12] Zhou D, Pan Q, Shen F, et al. Total fecal microbiota transplantation alleviates high-fat diet-induced steatohepatitis in mice via beneficial regulation of gut microbiota. Sci Rep,2017,7(1):1529.
[13] Motonaga K, Ota M, Odawara K, et al. A comparison of potency differences among thyroid peroxidase (TPO) inhibitors to induce developmental toxicity and other thyroid gland-linked toxicities in humans and rats. Regul Toxicol Pharmacol,2016,80:283-290.
[14] Akmal A, Kung J. Propylthiouracil, and methimazole, and carbimazole-related hepatotoxicity. Expert Opin Drug Saf,2014,13(10):1397-1406.
[15] Wang M T, Lee W J, Huang T Y, et al. Antithyroid drug-related hepatotoxicity in hyperthyroidism patients: a population-based cohort study. Br J Clin Pharmacol,2014,78(3):619-629.
[16] Dong L N, Wang J P, Liu P, et al. Faecal and mucosal microbiota in patients with functional gastrointestinal disorders: Correlation with toll-like receptor 2/toll-like receptor 4 expression. World J Gastroenterol,2017,23(36):6665-6673.
[17] Fornefett J, Krause J, Klose K, et al. Comparative analysis of humoral immune responses and pathologies of BALB/c and C57BL/6 wildtype mice experimentally infected with a highly virulent Rodentibacter pneumotropicus (Pasteurella pneumotropica) strain. BMC Microbiol,2018,18(1):45.
[18] Pan H, Guo R, Zhu J, et al. A gene catalogue of the Sprague-Dawley rat gut metagenome. Gigascience,2018,7(5):332-336.
[19] 骆庆玲,周永健. 粪菌移植治疗非酒精性脂肪性肝病研究与思考. 实用肝脏病杂志,2017,20(6):647-650.

Effects of fecal bacteria transplantation on microflora in rats with high-fat-induced liver steatosis

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